Oven, C.H. van

mylar culture particle source α particles protein mylar membrane irradiation method membrane microscope option array nature option b damage glass imaging americium source break repair response confocal microscope medium laser number image dsb response proteins dna damage irradiate procedure culture medium live-cell glass rings irradiate cells group culture dishes protocol fluorescence nucleus confocal silicone americium wide-field fluorescence microscope cell nucleus cell cultures cells 3 times publishing table culture dish α- particle irradiation single-strand dna breaks α- particle assay repair proteins dsb arrays dsb repair response dna double-strand breaks march coverslip setup stack troubleshooting wide-field experiment energy objective fluorescently diameter room temperature analysis immunodetection center mylar dishes metal holder mylar bottom genome cell types glow-discharged carbon genomic glass ring