Quinto, J.D.

assay sequence virus strain rt-pcr probe lineage prototype primers detection target sequences rna runoff transcripts prototype hmpv strains nl-n assay sample primer target number metapneumoviru panel result dilution mismatch alt-n alt-n assays infection transcript rt-pcr assay end-point rt-pcr runoff alt-n assay reaction disease sensitivity nucleoprotein gene rt-pcr assays value 5 end primer sets amplification children nucleoprotein taqman signal sequence information genbank database probe sequences hmpv sequences limit target sequence false-negative results cycle prototype strains netherland 2003. prototype viruses tract disease osterhau ct values tract microbiol end-point genbank entropy x axes hmpv prototype strains isolation metapneumovirus infection hmpv strains entropy plots basis hoogen specificity 50 rna copies pcr amplification fouchier oligonucleotide bioedit software package 1 min transcriptase pcr assay