Birkenhäger, J.C.

kh 1060 fragment effect vdr half-life ligand trypsin change ros 17/2.8 cells analog vdr conformation receptor protease van den bemd concentration half-life activity ug /ml 17/2.8 binding vehicle incubation room temperature cleavage sites sds /page affinity degradation protein study protection result vdr content conformation vdr fragments ligand binding domain tnt lysate assay temperature stabilization difference 32- kda fragment protease activity 24 hr biochemistry vdr stabilization binderup digestion vdr protein bone resorption /page deluca 32- -61 presence antibody level assay analysis response endocrinology thyroid hormone kh 1060-incubated cells biochem gain insight cell proliferation van leeuwen 3 analogs usa 93 biochemistry 24- kda fragment vitamin retinoic -90 vdr affinity vdr stability 10 min vdr level cleavage -35 trypsin concentration immunoblot analysis side chain molecule