Doorn, J.

medium factor growth bone formation ct-cm proliferation expression effect growth factors formation differentiation secretion tissue increase mesenchymal concentration cell types bmp -2 migration pathway bt-cm engineering 5000 cells /cm bone tissue engineering level treatment trophic 5 days proliferation medium huvec culture analysis 3 days protein nt-cm osteogenic application alp expression biomaterial growth factor secretion mesenchymal stromal cells assay atf 4 doorn stromal osteoblast marrow -63 osteogenic differentiation number antibody plate wound bone marrow mesenchymal result id 2 expression glucose speci id 1 expression mg -63s huvecs microarray technology control host cells 4 days trophic factors lactate concentrations bmpr-ia cell numbers van blitterswijk lactate bone-speci fluence camp-treated target system triplicate receptor presence activity