Agami, R.

mir -27a cyclin expression fbw 7 cyclin e protein cycle mirna reporter luciferase cancer i i c ds i t level analysis cell cycle control hek 293 cells microrna target activity cell cycle progression transfected cell lines mir -27a overexpression figure dna replication stress overexpression plasmid vector cyclin e protein 2os cells reference b-all oligo method fbw 7 protein fbw 7 expression inhibitor regulator pre-mir result progression mir 27a 48 hrs luciferase reporter experiments cycloheximide chase analysis cyclin e levels function pre-mir-control oligos regulation figure 1 c luciferase activity antibody s-phase flow cytometry replication degradation stress phase wild-type experiment material suppressor blot analysis pre-mir -27a reed si tumor study fbw 7. overexpressed ubiquitylation cell cycle phases transfection cytometry mir -27a expression qrt-pcr pre-mir -27a oligos g 1/s boundary repression