Silvennoinen, O.

protein peptide kinase domain jak 2 substrate jh 2 domain activity receptor microarray mutation analysis reaction jh 1-jh phosphorylation preference signal concentration value tyrosine figure peptide microarrays sequence activation interaction antibody substrate preference jak 2 proteins change specificity precursor difference activation loop v 617f mutation jh 1 domain study array vmax /km km values kinase activity pamchiph effect mechanism vmax /km values pgfrb volume incubation janus kinase 2 april position motif www.plosone.org issue result pseudokinase figure 1 buffer phosphorylated comparison efficiency protein kinases growth factor v 617f jak 2 activity inhibitor table increase 400 mm atp mutation v 617f jak proteins addition image point expression time series janus kinases method identification residue