Vries-Van Melle, M.L. de

cartilage defect osteochondral biopsy culture model repair osteochondral biopsies tissue 28 days cartilage-only explants explant chondrocyte subchondral bone repair tissue subchondral treatment expression osteochondral culture model cartilage-only depth medium articular collagen cartilage defects mechanism study culture medium osteochondral defects articular cartilage cartilage explants matrix osteoarthritis membrane autologous chondrocyte implantation osteochondral biopsy cartilage osteochondral biopsy model safranin-o staining calci fied cartilage seeding integration section activity microfracture vries-van melle cartilage repair scale bars matrix deposition adamt difference factor strategy autologous chondrocyte transplantation cartilage repair mechanisms aggrecanases adamts 4 result collagen type 1 collagen type 2 collagen type x articular cartilage repair calcein chondral histology volume van osch environment validation autologou effect wound implantation subchondral bone changes safranin-o staining mcp joints h &e staining fi xed procedure osteochondral biopsy bone safranin-o –stained sections