Ramnarain, S.

mutation patient receptor wild-type ar gene androgen protein expression analysis splice intron transcript index subject sequence plasmid subject absence probe 114- kd isoform index hormone androgen receptor control splice site dna binding presence primer position deletion binding fraction pcr-sscp analysis index patient family ar protein individual assay 110112- kd doublet transcription activation phenotype cluster androgen insensitivity 1 mm edta lanes 1 patient iii -2. androgen receptor gene brinkmann exon 3 van rooij hcj transcription region kuiper rt-pcr study method sequencing endocrinol splice-acceptor site insensitivity exons 2 psvar rt-pcr studies isoform lanes 3 table acid residues wild-type ar trapman j reaction genet kuiper ggjm pcr-sscp wild-type transcript zinc clusters androgen insensitivity syndrome buffer figure intronic 10 min centrifugation mean-fold induction sem