Jongmans, W.

vector clone plasmid library transfection selection system pecvh hprt cdna cell lines hatr clones expression cdna library sequence figure efficiency result acids research isolation interest number episomal 1150 bp pecvh 3-2 experiment research cell line transfected frequency transfection frequency transfection efficiency episome cdna inserts insert pecvh 2-2 hygromycin phenotype polyadenylation procedure fraction hypoxanthine model system dna uptake hypoxanthine phosphoribosyltransferase correction selection frequency ecori site 2-2 12roh cdna libraries sucrose gradient centrifugation k 562 cells ecori episomal vector system calf serum hprt mrna phenotypic correction xp 12roh cells alternative polyadenylation lesch-nyhan hoeijmaker month 3-2 subcloning region phenotypic medium bootsma westerveld culture generation ebv-derived microinjection transfectant pecv 25 xnmt-pcd orientation preparation transfectability population