Verbeek, E.

af /af mice clone af 508 mutation mouse mutation fibrosis figure response chloride cf patients fragment af 508 allele analysis forskolin af /af allele probe sequence selection patient procedure af /af mouse model ileum channel mouse model intestine sspi site conductance littermate gallbladder sspi restriction site tissue figures 6 exon 10 af 508/sspi site table processing abnormality transmembrane integration 5.7 kb probe b basal regulator table ii product crypt genomic af 508 mice control 2.9 riordan g 418 resistance gluconate activity passage af 508 cftr electrophysiological abnormalities mucus probe c af 508 cf mice hybridized cftr gene colony forskolin response figure ic epithelium epithelia bradley grubb chloride permeability g 418 table i plasmid sequences obstruction duplication disease linearization