Huisman, W.M.

specimen inhibition amplicor trachomati inhibition rate sample heat treatment chlamydia trachomatis dilution 10- chlamydia study procedure processing patient amplicor c transport assay pretreatment polymerase chain reaction 2 sp specimen cell culture amplicor specimen amplicor transport buffer treatment detection pretreatment procedure blood contamination 10 min effect trachomatis pcr assay microbiol protocol blood endocervical specimens reaction amplicor pcr culture sensitivity factor polymerase medium endocervical roche manufacturer result chain room temperature alternative transport medium buffer inhibition rates concentration 100 ifu infection target dna concentration 2 sp specimens method microbiology outpatient clinic freeze-thawing diagnosis 1993. target women netherland contamination evaluation trachomatis pcr 100 l ph values collection amplicor protocol prevalence pcr inhibitors inhibitor trachomatis infections society system pretreatment methods pcr inhibition