Sixma, T.K.

binding domain protein triad nucleotide triad 1 mismatch state formation ubiquitin presence magnesium structure figure repair residue dimer atpase chain interaction absence ligase rad 18 937 cells table rad 6b release cross-link variant subunit concentration assay affinity mismatch-binding domain experiment model analysis cysteine hydrolysis erasmus mc study nucleotide binding activity atp binding dimers rad 18 ring cross-linking enzyme type muts addition result number ubch 7 connector domain amp.pnp molecule chemical ubiquitination buffer downloaded ubc 13 connector change cross-link formation dna mismatch mutl -131c transduced asymmetry fluor mismatch-binding muts -246c atp hydrolysis 693n muts chemistry difference position muts-mutl spectra alexa growth