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virus disul fide bonds disul protein vector mutant figure expression replication gp 120 hcv replication shrna ns 1 proteins activity strain mandrill function influenza figure 3 class sivmnd lentiviral sequence control rss activity disul fide bond cysteine virol luciferase promoter effect ns 1 protein signal hek 293t cells library ns 1 genes culture envelope wild-type assay ficiency level infection glycoprotein chase signal peptide transduced sivdrl peptide species gp 160 immunode medium inhibition mirna puromycin resistance gene class 2 -122 off-target off-target effects drill primer signal peptide cleavage sample maturation suppression mandrillus sphinx ifn-b factor monkey number puromycin mandrillu hepatitis lentiviral shrna libraries van der laan signi study supt 1 cells sphinx