Busslinger, M.

methylated fragment residue sequence hpaii clone ccgg sites c residue cleavage haeiii research unmethylated enzyme restriction l cells methylation hemimethylated phage result acids research dna methylation psti fragment methylase a-globin hindiii c residues hpaii methylase methylated dna presence digestion hemimethylated dna ay-globin gene y-globin y-globin gene globin probe unmethylated dna a-globin gene hemimethylated myl dna agarose ccgg site ay-globin globin genes al-globin gene hemimethylated a-globin genes unmethylated a-globin dna methyl strand modification hybridized vector flavell al-globin bottom part restriction enzymes mc residue gunthert clones 2 mc residues calf serum failure size marker weight dna dna fragments experiment l-cell pattern methyl group cell lines dna sequence stein transformation sequence ggcmcgg lanes show salmon sperm dna phage 1 dna methylation-sensitive enzymes msi sequence ggc cgg u.s.a mspi incubation conditions