Hiyama, H.

protein hhr 23 proteins hhr 23b proteasome interaction domain rhhr 23b yeast nickel-chelating sepharose beads ub-like ub-like domain panel ubiquitin sequence buffer rs 5a extract beads binding antibody region ub-like sequence homolog 125 i-lysozyme degradation nh 2 terminus degradation fraction presence result immunoblotting erasmus mc deletion sepharose i-lysozyme 0.25 mm pmsf panel b terminus 2 mm atp rabbit reticulocyte extracts rhhr 23 proteins rhhr 23b proteins deletion mutants cell extracts activity his-tagged reticulocyte amersham pharmacia rhhr 23a 2-terminal nickel-chelating glycerol library ub-rhhr 23b mutant reticulocyte extracts 125 i-lysozyme multiubiquitin two-hybrid amount rad 23 buffer h gradient proteasome activity 5a protein lane 1. sds sample buffer glycerol gradient centrifugation acids nh 2-terminal rad 23 homologs experiment rnedd 8 6 hhr 23b protein serum albumin subunit multiubiquitin chain panel c function cooh terminus erasmu gvdp tetrapeptide