Effects of 16S rRNA gene mutations on tetracycline resistance in Helicobacter pylori
January 2003
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The triple-base-pair 16S rDNA mutation AGA(926-928)-->TTC mediates high-level tetracycline resistance in Helicobacter pylori. In contrast, single- and double-base-pair mutations mediated only low-level tetracycline resistance and decreased growth rates in the presence of tetracycline, explaining the preference for the TTC mutation in tetracycline-resistant H. pylori isolates.
Keywords
- Cloning, Molecular
- DNA Primers
- Reverse Transcriptase Polymerase Chain Reaction
- Mutagenesis, Site-Directed
- Helicobacter pylori/*drug effects/*genetics/growth & development
- Mutation/*genetics/*physiology
- RNA, Ribosomal, 16S/*genetics
- Tetracycline Resistance/*genetics/*physiology
Automatically Extracted Terms
- mutation
- tetracycline
- tetracycline resistance
- pylori
- resistance
- mutant
- helicobacter pylori
- strain
- helicobacter
- rrna genes
- double-base-pair mutations
- sequence
- growth
- rrna gene
- position
- agents chemother
- agc mutants
- rrna gene mutations
- positions 926
- growth rates
