Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit
May 2006
Article
volume 65, issue 2 pp 354-356.
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The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.
Keywords
- Humans
- Sensitivity and Specificity
- Carrier State/*microbiology
- Polymerase Chain Reaction/*methods
- *Reagent Kits, Diagnostic
- Nasal Cavity/*microbiology
- Staphylococcus aureus/genetics/*isolation & purification
- Staphylococcal Infections/*diagnosis/microbiology
