Isolation of human oncogene sequences (v-fes homologue) from a cosmid library.
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To define the human homolog (or homologs) of transforming sequences (v-fes gene) common to Gardner (GA) and Snyder Theilen (ST) isolates of feline sarcoma virus (FeSV), a representative library of human lung carcinoma DNA in a cosmid vector system was constructed. Three cosmid clones were isolated containing GA/ST FeSV v-fes homologous cellular sequences, within 32- to 42-kilobase cellular inserts representing 56 kilobases of contiguous human cellular DNA. Sequences both homologous to, and colinear with, GA or ST FeSV v-fes are distributed discontinuously over a region of up to 9.5 kilobases and contain a minimum of three regions of nonhomology representing probable introns. A thymidine kinase selection system was used to show that, upon transfection to RAT-2 cells, the human c-fes sequence lacked detectable transforming activity.
- Support, Non-U.S. Gov't
- Support, U.S. Gov't, P.H.S.
- Escherichia coli/genetics
- Genes, Viral
- 0 (DNA, Recombinant)
- DNA, Recombinant
- EC 3.1.21 (DNA Restriction Enzymes)
- Bacteriophage lambda/genetics
- Cell Transformation, Viral
- Cloning, Molecular/methods
- DNA Restriction Enzymes/diagnostic use