The sequence GGCmCGG is resistant to MspI cleavage
January 1983
Article
volume 11, issue 11 pp 3559-3569.
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MspI essentially fails to cut the sequence GGCmCGG at enzyme concentrations which give total digestion of CCGG, CmCGG and GGCCGG sites. This result explains why certain sites in mammalian DNA are resistant to both MspI and HpaII and shows that this results from an idiosynchracy of MspI rather than a novel form of DNA methylation at this site in mammalian cells.
Keywords
- Animals
- 9007-49-2 (dna)
- Human
- Support, Non-U.S. Gov't
- Cloning, Molecular
- Mice
- Base Sequence
- Substrate Specificity
- Nucleic Acid Hybridization
- Genes, Structural
- Dna
- Methylation
- EC 3.1.21 (DNA Restriction Enzymes)
- DNA Restriction Enzymes/metabolism
- 9004-22-2 (Globins)
- Globins/genetics
- Deoxyribonuclease HpaII
- EC 2.7.1.21 (Thymidine Kinase)
- EC 3.1.21.- (Deoxyribonuclease HpaII)
- L Cells (Cell Line)/enzymology
- Thymidine Kinase/deficiency
Automatically Extracted Terms
- methylated
- fragment
- sequence
- residue
- hpaii
- clone
- ccgg sites
- c residue
- cleavage
- haeiii
- unmethylated
- restriction
- research
- enzyme
- phage
- methylation
- l cells
- hemimethylated
- result
- psti fragment
