http://hdl.handle.net/1765/2544




Transcription factor Sp1 is essential for early embryonic development but dispensable for cell growth and differentiation.


Article
volume 89, issue 4 pp 619-628.
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Transcription factor Sp1 has been implicated in the expression of many genes. Moreover, it has been suggested that Sp1 is linked to the maintenance of methylation-free CpG islands, the cell cycle, and the formation of active chromatin structures. We have inactivated the mouse Sp1 gene. Sp1-/- embryos are retarded in development, show a broad range of abnormalities, and die around day 11 of gestation. In Sp1-/- embryos, the expression of many putative target genes, including cell cycle-regulated genes, is not affected, CpG islands remain methylation free, and active chromatin is formed at the globin loci. However, the expression of the methyl-CpG-binding protein MeCP2 is greatly reduced in Sp1-/- embryos. MeCP2 is thought to be required for the maintenance of differentiated cells. We suggest that Sp1 is an important regulator of this process.



Keywords


Automatically Extracted Terms
  • embryo
  • figure
  • protein
  • expression
  • island
  • / embryos
  • mecp 2
  • sp 1 gene
  • methylation
  • mouse
  • mecp 2 protein
  • cpg islands
  • / es cells
  • development
  • binding
  • analysis
  • es cells
  • transcription
  • hpaii
  • aprt gene


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