http://hdl.handle.net/1765/2550




CLIP-115, a novel brain-specific cytoplasmic linker protein, mediates the localization of dendritic lamellar bodies.


Article
volume 19, issue 6 pp 1187-1199.
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Intracellular localization of organelles may depend in part on specific cytoplasmic linker proteins (CLIPs) that link membranous organelles to microtubules. Here, we characterize rat cDNAs encoding a novel, brain-specific CLIP of 115 kDa. This protein contains two N-terminal microtubule-binding domains and a long coiled-coil region; it binds to microtubules and is homologous to CLIP-170, a protein mediating the binding of endosomes to microtubules. CLIP-115 is enriched in the dendritic lamellar body (DLB), a recently discovered organelle predominantly present in bulbous dendritic appendages of neurons linked by dendrodendritic gap junctions. Local microtubule depolymerization leads to a temporary reduction of DLBs. These results suggest that CLIP-115 operates in the control of brain-specific organelle translocations.



Keywords


Automatically Extracted Terms
  • clip -115
  • -115
  • protein
  • microtubule
  • figure
  • domain
  • cos 1 cells
  • neuron
  • antiserum
  • brain
  • organelle
  • dendrodendritic gap junctions
  • mtb domains
  • antibody
  • dendritic
  • clone
  • nocodazole
  • density
  • clip -170
  • antisera


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