CFU-S(11) activity does not localize solely with the aorta in the aorta-gonad-mesonephros region
January 2000
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The aorta-gonad-mesonephros (AGM) region is a potent hematopoietic site in the midgestation mouse conceptus and first contains colony-forming units-spleen day 11 (CFU-S(11)) at embryonic day 10 (E10). Because CFU-S(11) activity is present in the AGM region before the onset of hematopoietic stem cell (HSC) activity, CFU-S(11) activity in the complex developing vascular and urogenital regions of the AGM was localized. From E10 onward, CFU-S(11) activity is associated with the aortic vasculature, and is found also in the urogenital ridges (UGRs). Together with data obtained from organ explant cultures, in which up to a 16-fold increase in CFU-S(11) activity was observed, it was determined that CFU-S(11) can be increased autonomously both in vascular sites and in UGRs. Furthermore, CFU-S(11) activity is present in vitelline and umbilical vessels. This, together with the presence of CFU-S(11) in the UGRs 2 days before HSC activity, suggests both temporally and spatially distinct emergent sources of CFU-S(11). (Blood. 2000;96:2902-2904)
- Male
- Animals
- Female
- Research Support, Non-U.S. Gov't
- Mice
- Research Support, U.S. Gov't, P.H.S.
- Mice, Inbred C57BL
- Mice, Inbred CBA
- Crosses, Genetic
- Gestational Age
- Cell Lineage
- Hematopoietic Stem Cells/*cytology
- Microscopy, Electron, Scanning
- *Hematopoiesis, Extramedullary
- *Mesonephros
- Animals, Outbred Strains
- Aorta/*embryology
- Gonads/*embryology
- Radiation Chimera
- cfu-s 11
- aorta
- cfu-s 11 activity
- activity
- number
- tissue
- embryo
- region
- hematopoietic
- agm region
- increase
- culture
- panel
- mouse
- cell counts
- figure
- explant
- dzierzak
- adult
- dzierzak e
