Nucleotide excision repair (NER) is a versatile DNA repair pathway that removes a wide range of bulky DNA lesions. It is a complex mechanism requiring the action of at least 30 proteins. It is essential that each step is accurately orchestrated and tightly regulated to ensure efficient repair and maintenance of genomic integrity. Since its discovery more than fifty years ago, NER is already heavily-studied. However current understanding about its spatio-temporal regulation is still limited. In this thesis, we aimed to identify novel NER regulators and found two new factors, HLTF and FACT subunit Spt16, respectively. We report the former to be required for removal of incised DNA fragment including the lesion, and latter to be required for recruitment of TC-NER factor UVSSA to lesion.

DNA damage response, DNA repair, Nucleotide Excision Repair, TFIIH, proteomics, microscopy
W. Vermeulen (Wim) , J.A. Marteijn (Jurgen)
Erasmus University Rotterdam
Department of Molecular Genetics

Türkyilmaz, Y. (2020, July 3). A quest to reveal novel players in nucleotide excision repair: From proteomics to mechanistic insights. Erasmus University Rotterdam. Retrieved from