%PDF-1.7
%
134 0 obj
<>
endobj
123 0 obj
<>
endobj
133 0 obj
<>stream
Acrobat Distiller 8.1.0 (Windows)
2020-06-26T11:37:28+08:00
Arbortext Advanced Print Publisher 9.1.440/W Unicode
2020-07-22T01:36:11-07:00
2020-07-22T01:36:11-07:00
uuid:b7bd8776-77a4-4556-b23c-6595aaf8c147
uuid:0a1534c7-1dd2-11b2-0a00-af00c876edff
application/pdf
Science Journals — AAAS
endstream
endobj
120 0 obj
<>
endobj
2 0 obj
<>
endobj
122 0 obj
<>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageC]/Properties<>/XObject<>>>/Rotate 0/Thumb 117 0 R/Type/Page>>
endobj
1 0 obj
<>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageC]/Properties<>/XObject<>>>/Rotate 0/Thumb 3 0 R/Type/Page>>
endobj
66 0 obj
<>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/XObject<>>>/Rotate 0/Thumb 67 0 R/Type/Page>>
endobj
76 0 obj
<>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageC]/Properties<>/XObject<>>>/Rotate 0/Thumb 77 0 R/Type/Page>>
endobj
100 0 obj
<>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/XObject<>>>/Rotate 0/Thumb 101 0 R/Type/Page>>
endobj
219 0 obj
<>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/Type/Page>>
endobj
239 0 obj
[245 0 R 246 0 R 247 0 R 248 0 R 249 0 R 250 0 R 251 0 R 252 0 R 253 0 R 254 0 R 255 0 R 256 0 R 257 0 R 258 0 R]
endobj
240 0 obj
<>stream
q
87.5 0 0 21.75 72 662.25 cm
/Im0 Do
Q
BT
/T1_0 1 Tf
10 0 0 10 72 622 Tm
(SARS-CoV-2 productively infects human gut enterocytes)Tj
ET
BT
/T1_1 1 Tf
8 0 0 8 72 584.99991 Tm
(and Hans Clevers)Tj
0 1 TD
(Schipper, Willine J. van de Wetering, Miranda de Graaf, Marion Koopmans,\
Edwin Cuppen, Peter J. Peters, Bart L. Haagmans)Tj
0 1.00001 TD
(Paul van Schayck, Anna Z. Mykytyn, Hans Q. Duimel, Elly van Donselaar, S\
amra Riesebosch, Helma J. H. Kuijpers, Debby )Tj
0 1 TD
(Mart M. Lamers, Joep Beumer, Jelte van der Vaart, K\350vin Knoops, Jens \
Puschhof, Tim I. Breugem, Raimond B. G. Ravelli, J.)Tj
ET
BT
/T1_1 1 Tf
8 0 0 8 181.83997 538.99997 Tm
(originally published online May 1, 2020)Tj
-13.73 0 Td
(DOI: 10.1126/science.abc1669)Tj
5.50301 1 Td
( \(6499\), 50-54.)Tj
/T1_0 1 Tf
-1.668 0 Td
(369)Tj
/T1_2 1 Tf
-3.83501 0 Td
(Science\240)Tj
ET
BT
/T1_1 1 Tf
8 0 0 8 145.45605 434.99973 Tm
(, this issue p. 50)Tj
/T1_2 1 Tf
-3.55701 0 Td
(Science)Tj
/T1_1 1 Tf
-3.125 1 Td
(gut.)Tj
0 1.00001 TD
(demonstrates that intestinal organoids can serve as a model to understan\
d SARS-CoV-2 biology and infectivity in the )Tj
8.53298 1 Td
(resulting in the production of large amounts of infective virus particle\
s in the intestine. This work)Tj
/T1_3 1 Tf
-1.09799 0 Td
(--)Tj
/T1_1 1 Tf
-6.33699 0 Td
(the enterocyte)Tj
/T1_3 1 Tf
-1.098 0 Td
(--)Tj
/T1_1 1 Tf
T*
(''mini-gut'' cultured in a dish, to demonstrate that SARS-CoV-2 readily \
replicates in an abundant cell type in the gut lining)Tj
35.40396 1 Td
( used human intestinal organoids, a )Tj
/T1_2 1 Tf
-2.16799 0 Td
(et al.)Tj
/T1_1 1 Tf
-33.23597 0 Td
(express the receptor that SARS-CoV-2 uses to gain entry to cells. Lamers\
)Tj
0 1 TD
(vomiting, and abdominal pain. Moreover, the virus has been detected in a\
nal swabs, and cells in the inner-gut lining)Tj
0 1.00001 TD
(respiratory transmission route; however, patients often present with gas\
trointestinal symptoms such as diarrhea, )Tj
3.125 1 Td
(Severe acute respiratory syndrome coronavirus 2 \(SARS-CoV-2\) causes an\
influenza-like disease with a)Tj
/T1_0 1 Tf
-3.125 1 Td
(Intestinal organoids as an infection model)Tj
ET
BT
/T1_0 1 Tf
6 0 0 6 79 369 Tm
(ARTICLE TOOLS)Tj
ET
BT
0 0 1 rg
/T1_1 1 Tf
8 0 0 8 187 367 Tm
(http://science.sciencemag.org/content/369/6499/50)Tj
ET
BT
0 g
/T1_0 1 Tf
6 0 0 6 79 326.99997 Tm
(MATERIALS)Tj
T*
(SUPPLEMENTARY )Tj
ET
BT
0 0 1 rg
/T1_1 1 Tf
8 0 0 8 187 331 Tm
(http://science.sciencemag.org/content/suppl/2020/04/30/science.abc1669.D\
C1)Tj
ET
BT
0 g
/T1_0 1 Tf
6 0 0 6 79 284.99997 Tm
(CONTENT)Tj
T*
(RELATED )Tj
ET
BT
0 0 1 rg
/T1_1 1 Tf
8 0 0 8 187 232.99979 Tm
(http://stm.sciencemag.org/content/scitransmed/12/550/eabc3539.full)Tj
0 1 TD
(http://stm.sciencemag.org/content/scitransmed/12/549/eabb9401.full)Tj
0 1.00001 TD
(http://stm.sciencemag.org/content/scitransmed/12/546/eabc1931.full)Tj
0 1 TD
(http://stm.sciencemag.org/content/scitransmed/9/396/eaal3653.full)Tj
0 1.00001 TD
(http://stm.sciencemag.org/content/scitransmed/12/541/eabb5883.full)Tj
0 1 TD
(http://immunology.sciencemag.org/content/immunology/5/47/eabc3582.full)Tj
T*
(http://stm.sciencemag.org/content/scitransmed/12/534/eabb1469.full)Tj
0 1.00001 TD
(http://stm.sciencemag.org/content/scitransmed/11/499/eaat0360.full)Tj
ET
BT
0 g
/T1_0 1 Tf
6 0 0 6 79 217 Tm
(REFERENCES)Tj
ET
BT
0 0 1 rg
/T1_1 1 Tf
8 0 0 8 187 206.99997 Tm
(http://science.sciencemag.org/content/369/6499/50#BIBL)Tj
0 g
0 1 TD
(This article cites 41 articles, 11 of which you can access for free)Tj
ET
BT
/T1_0 1 Tf
6 0 0 6 79 181 Tm
(PERMISSIONS)Tj
ET
BT
0 0 1 rg
/T1_1 1 Tf
8 0 0 8 187 179 Tm
(http://www.sciencemag.org/help/reprints-and-permissions)Tj
ET
BT
/T1_1 1 Tf
8 0 0 8 193.38391 120 Tm
(Terms of Service)Tj
0 g
-15.17299 0 Td
(Use of this article is subject to the )Tj
ET
72 106 m
521 106 l
0 0 m
S
BT
ET
BT
/T1_1 1 Tf
8 0 0 8 357.45605 85.99997 Tm
( is a registered trademark of AAAS.)Tj
/T1_2 1 Tf
-3.55701 0 Td
(Science)Tj
/T1_1 1 Tf
-32.125 0 Td
(Science, 1200 New York Avenue NW, Washington, DC 20005. The title )Tj
3.83501 1 Td
(\(print ISSN 0036-8075; online ISSN 1095-9203\) is published by the Amer\
ican Association for the Advancement of)Tj
/T1_2 1 Tf
-3.83501 0 Td
(Science )Tj
ET
BT
/T1_1 1 Tf
8 0 0 8 72 72 Tm
(Copyright \251 2020, American Association for the Advancement of Science\
)Tj
ET
BT
/T1_1 1 Tf
0 -8 8 0 575 319.76013 Tm
(on July 22, 2020)Tj
-0.278 0 Td
( )Tj
0 0 1 rg
-13.50598 0 Td
(http://science.sciencemag.org/)Tj
0 g
-8.11398 0 Td
(Downloaded from )Tj
ET
endstream
endobj
244 0 obj
<>/Filter/FlateDecode/Height 87/Length 19684/Name/X/Subtype/Image/Type/XObject/Width 350>>stream
HyTS߽Ia
"6p\P縠x:Z.Suk:8ud,EԺУ:xtSVk9@na}/ Ҟ6Gy@{_rcL"bFSgjz"UH}>2Z
5f @a,uWP24
jѨ\i-~==Op(rw]EQڇMyƧH4E5(HQ_-*P1.#*kb"f_+XA¾ՆK$FxUFJeFq {vdcg8ZS#3_kX `9'p (3u_Od}uvn|TSI#
!y 3|].Fh{U_JA-M0bD22}[-(U~[yTk|H mL;ƶc 0X ?1Ar?oƀU]C:/^ّ,7(a"Gq?b#QUaS L6)$Hm[ANc tPĎvURl2!VV{^e(^I9Dã3l]]\3$!i;v{?.Ҕ}31,ER[^k4Nok#b7fIȐy`au^GUĉ
W&\y,$d<|rD;VӪn>Mј[Mi#DQ LY2Cme#BV~2`ls~s W&
=xAJE^dɡ>S=`_dgk+gqaنKgbK=E[> @AQbg(