An Atherogenic Diet Disturbs Aquaporin 5 Expression in Liver and Adipocyte Tissues of Apolipoprotein E-Deficient Mice: New Insights into an Old Model of Experimental Atherosclerosis

The dysfunction of vascular endothelial cells is profoundly implicated in the pathogenesis of atherosclerosis and cardiovascular disease, the global leading cause of death. Aquaporins (AQPs) are membrane channels that facilitate water and glycerol transport across cellular membranes recently implicated in the homeostasis of the cardiovascular system. Apolipoprotein-E deficient (apoE−/−) mice are a common model to study the progression of atherosclerosis. Nevertheless, the pattern of expression of AQPs in this atheroprone model is poorly characterized. In this study, apoE−/− mice were fed an atherogenic high-fat (HF) or a control diet. Plasma was collected at multiple time points to assess metabolic disturbances. At the endpoint, the aortic atherosclerotic burden was quantified using high field magnetic resonance imaging. Moreover, the transcriptional levels of several AQP isoforms were evaluated in the liver, white adipocyte tissue (WAT), and brown adipocyte tissue (BAT). The results revealed that HF-fed mice, when compared to controls, presented an exacerbated systemic inflammation and atherosclerotic phenotype, with no major differences in systemic methylation status, circulating amino acids, or plasma total glutathione. Moreover, an overexpression of the isoform AQP5 was detected in all studied tissues from HF-fed mice when compared to controls. These results suggest a novel role for AQP5 on diet-induced atherosclerosis that warrants further investigation.


Introduction
Despite significant advances in the treatment of cardiovascular disease (CVD), it remains the leading cause of mortality and morbidity among adults [1]. The major cause of CVD is atherosclerosis that is elicited by the early impairment of endothelial function and results in a chronic inflammatory condition in which arteries harden through the build-up of lipid-rich plaque in the vessel wall [2]. magnetic resonance imaging (MRI). After confirming a strong atherosclerotic phenotype in the HF-fed mice, we determined the mRNA expressions of the orthodox isoforms, AQP1 and AQP5, and of the aquaglyceroporins AQP3, AQP7, and AQP9, in different metabolic tissues (BAT, WAT, and liver).

Animals and Diets
Seven-week-old male apoE −/− mice (Jackson Laboratory, Bar Harbor, ME, USA) were fed one of the following diets prepared based on AIN 93M recommendations but with different levels of fat/cholesterol (Research Diets, New Brunswick, NJ, USA). A control diet (C, 5% w/w fat) or a High Fat diet (HF, 20% w/w fat and 0.15% cholesterol). Macronutrient dietary composition is shown in Table A1. Animals were housed in a room at 22 ± 2 • C with a 12-h light-dark cycle with free access to food and water during 14 (±2) weeks. Diets were refreshed weekly, at which time animals and their remaining food were weighed. Food consumption was estimated as an average per mouse per day within each cage. All procedures were performed in compliance with the Institutional Animal Care and Use Committee of the Pennsylvania State University, which specifically approved this study.

Blood Collection
At different time points, blood was collected from the retroorbital sinus of anesthetized animals into heparinized tubes and immediately put on ice. Within 30 min, plasma was isolated by centrifugation at 2000 rpm, 15 min, at 4 • C and stored at −80 • C prior to further biochemical analyses. Due to the limited volume of blood obtained, samples obtained at different time-points were used in the subsequent biochemical analysis, as further detailed.

Systemic Methylation Index
The effect of 8 weeks of experimental diets on systemic methylation index was evaluated by the ratio of the plasmatic concentrations of S-adenosylmethionine (AdoMet) to S-adenosylhomocysteine (AdoHcy) (AdoMet/AdoHcy ratio) quantified by liquid chromatography tandem-mass spectrometry (LC-MS/MS), as previously described [30].

Triacylglycerols
The effect of 10 weeks of experimental diets on the fasting plasma concentrations of TAG were measured using a colorimetric assay (Cayman, Ann Arbor, MI, USA) following the manufacturer's protocol.

Plasma Amino Acids and Glutathione
Plasma concentrations of amino acids and total glutathione after 12 weeks on each diet were quantified by adequate methodology. Specifically, amino acids were determined by gas chromatography-flame ionization detector (GC-FID) using the Phenomenex EZ:faastTM kit for physiological amino acid analysis (Phenomenex, Torrance, CA, USA) according to the manufacturer's instructions and as previously described in detail [31]. Total glutathione, defined as the total concentration of glutathione after reductive cleavage of all disulfide bonds, was quantified by high-performance liquid chromatography (HPLC) analysis with fluorometric detection, as previously described [32].

Glucose
At the end of each experiment, fasting blood glucose levels were determined using a glucometer (Contour, Bayer, Tarrytown, NY, USA) following the manufacturer's instructions.

Tissue Collection
At the end-point mice were euthanized by carbon dioxide inhalation and aortas were collected as previously described in detail [33]. Inguinal subcutaneous WAT and interscapular BAT were removed and immediately snap-frozen in liquid nitrogen. Livers were removed, weighted, sampled, and embedded in Optimal Cutting Temperature (OCT) compound (Sakura Finetek, Torrance, CA, USA) or immediately snap-frozen in liquid nitrogen. All samples were stored at −80 • C before further analyses.

Oil Red O Staining
Oil Red O staining was used to determine the distribution of lipid droplets in the liver. The OCT-frozen samples were cut into 8-µm-thick sections, fixed in 4% formaldehyde (Thermo Fisher Scientific, Waltham, MA, USA) for 10 min, and rinsed in deionized water and dried at room temperature before Oil Red O staining (Sigma-Aldrich, St. Louis, MO, USA). The sections were stained for 20 min with Oil Red O and rinsed in tap water for 5 min. Hematoxylin (Sigma-Aldrich, St. Louis, MO, USA) was counterstained to assist tissue visualization, and the slides were stained for 1 min and rinsed in tap water for 5 min. All of the representative images are at 400X magnification. The detailed information was described previously [34].

RNA Extraction
Total RNAs from WAT, BAT, and liver were extracted using the Qiagen RNeasy lipid tissue mini kit and Qiagen RNeasy mini kit, respectively (Qiagen, Germantown, MD, USA) and were stored at −80 • C prior to analysis. To exclude possible DNA contamination, the optional on-column DNA digestion with the RNase-free DNase (Qiagen) was performed during RNA extraction. All procedures were conducted by following the manufacturer's protocol. The RNA concentrations were determined spectrophotometrically at 260 nm using the NanoDrop 2000c (ThermoFisher Scientific, Waltham, MA, USA). The 260/280 nm ratio was utilized to assess the purity of RNA samples. Only samples with 260/280 nm ratios between 1.8 and 2.2 were used for cDNA synthesis. Additionally, agarose bleach gels were used to qualitatively assess RNA integrity by visualization of the 28S and 18S rRNA bands [35]. To generate cDNA for quantitative PCR, 500 ng of total RNA was reverse-transcribed for 1 h at 42 • C using M-MLV reverse transcriptase (Promega, Madison, WI, USA) and oligo(dT)15 Primer (Promega, Madison, WI, USA).

Quantification of Aortic Plaque Volume
Aortas were processed as previously described in detail [33]. Briefly, dissected aortas were equilibrated to 0.1% Magnevist (Bayer HealthCare Pharmaceuticals Inc., Whippany, NJ, USA), 0.25% sodium azide, PBS solution overnight at 4 • C, and plaque volume was determined by MRI using an Agilent 14T micro imaging system (Agilent Technologies, Inc., Santa Clara, CA, USA). After acquisition MR data was reconstructed using Matlab (The Mathworks Inc., Natick, MA, USA) and data segmentation was performed using Avizo 9.5 (Themo Fisher Scientific, Waltham, MA, USA). The lumen of the aorta, the different plaques, and the aorta wall were manually segmented. Quantification of plaque volume was obtained using the material statistics function on the segmented aorta and the results were expressed as the % of plaque volume in relation to the total segmented volume.

Statistical Analysis
Statistical analyses were performed in GraphPad Prism 7 (GraphPad Software, La Jolla, CA, USA), with statistical significance set to p < 0.05. For two-group comparison, an unpaired Student's t-test was used. For more than two groups, a one-analysis of variance (ANOVA) was performed, followed by Tukey's.

Results and Discussion
To investigate whether a HF diet would disturb the expression of AQPs in an atherosclerosis prone model, we fed apoE −/− mice HF or control diets. Only male mice were included to control for the known effect of gender on atherosclerosis in this strain [38].

Diet Consumption, Body and Liver Weights, and Liver Histology
The results showed that HF mice consumed significantly less food than the mice fed a control diet ( Figure 1A), nevertheless due to the higher energy density of the HF diet, more calories were consumed by HF-fed mice versus controls ( Figure 1B). As expected, HF mice gained more weight compared to controls [39,40] ( Figure 1C). No differences were observed in absolute liver weights (g) between the two groups of mice. The values (mean ± SEM, n = 10/group) were 1.17 ± 0.09 and 1.22 ± 0.13, for controls and HF, respectively. The relative liver weights were also similar in the two groups ( Figure 1D). Oil Red O staining showed that livers from HF-fed mice presented more macrovesicular lipid droplets of Oil Red O positive staining than controls ( Figure 2). Ballooned hepatocytes and sinusoids capillarization, on the other hand, were also exacerbated in the HF-fed mice, showing more inflated hepatocytes, as compared to the control mice, indicating liver damage by the HF diet. This finding is consistent with a previous study, in Oil Red O staining showed that livers from HF-fed mice presented more macrovesicular lipid droplets of Oil Red O positive staining than controls ( Figure 2). Ballooned hepatocytes and sinusoids capillarization, on the other hand, were also exacerbated in the HF-fed mice, showing more inflated hepatocytes, as compared to the control mice, indicating liver damage by the HF diet. This finding is consistent with a previous study, in which hepatic fat accumulation was reported in apoE −/− mice after seven weeks of a diet containing similar amounts of fat and cholesterol as the HF-diet used in this study [41]. Oil Red O staining showed that livers from HF-fed mice presented more macroves ular lipid droplets of Oil Red O positive staining than controls ( Figure 2). Balloon hepatocytes and sinusoids capillarization, on the other hand, were also exacerbated in HF-fed mice, showing more inflated hepatocytes, as compared to the control mice, in cating liver damage by the HF diet. This finding is consistent with a previous study, which hepatic fat accumulation was reported in apoE −/− mice after seven weeks of a d containing similar amounts of fat and cholesterol as the HF-diet used in this study [41]

Blood Biochemistry
We next evaluated the effect of the HF diet on several biochemical parameters. The results showed that the mice fed a HF diet had significantly increased fasting plasma TAG ( Figure 3A) and glucose levels ( Figure 3B), thus confirming the major metabolic disturbances elicited by the HF diet previously described by others [31,41,42]. In fact, male apoE −/− mice fed HF-diet are a widely used model of insulin resistance [43], a condition where aquaglyceroporins expression and function are affected [15,44,45].

Systemic Methylation Index
The ratio of the metabolites, S-adenosylmethionine (AdoMet) to S-adenosylhomocysteine (AdoHcy), or AdoMet/AdoHcy measures the cell methylation potential [46][47][48]. AdoMet is the universal methyl donor to cellular methyltransferases, originating the methylated substrate and the metabolite AdoHcy. Importantly, AdoHcy may negatively regulate the activity of those same methyltransferases. Thus, a decreased AdoMet/AdoHcy ratio reflects a hypomethylating environment. In previous cell studies we have observed that a decrease in AdoMet/AdoHcy ratio downregulated AQP1 expression and promoted an atherogenic endothelial phenotype [14]. Yun et al. have reported that a diminished methylation index was present in wild type mice fed HF diets [49]. These observations led us to measure the systemic concentrations of AdoMet and AdoHcy in this study. Nevertheless, the results revealed similar plasmatic AdoMet/AdoHcy ratios in both groups of animals, thus showing that the systemic methylation index was not affected by the HF diet ( Figure 3C) in apoE −/− mice.

Plasma Glutathione
The role of AQPs as facilitators of hydrogen peroxide membrane permeation, a major reactive oxygen species (ROS), has been recently reported [50]. Importantly, ROS build-up is a main pathophysiological mechanism favoring the establishment and progression of atherosclerosis [47]. Glutathione is a major cellular antioxidant that neutralizes hydrogen peroxide to water. Thus, we measured the systemic levels of total glutathione in our animals. The results, shown in Figure 3D, revealed similar plasma levels of glutathione in both groups of animals thereby suggesting that the bioavailability of this tripeptide is intact in the HF-mice. In support of this possibility, the plasma levels of three amino acids that form glutathione, i.e., glutamate ( Figure 3E), glycine ( Figure 3E), and cysteine (data not shown) did not differ between these two diets. We do acknowledge however that the measurement of the concentrations of both the free and reduced glutathione forms would be necessary to give a functional measure of this major antioxidant system.

Plasma Amino Acids
Next, we evaluated the plasmatic levels of amino acids to visualize any changes driven by the HF diet that could suggest disturbed metabolic pathways to be further related with diet-induced effects on AQPs expression. In fact, recent developments have begun to shed light on associations between compromised cardiometabolic function and altered intermediary metabolism of amino acids [51]. In the present study, the plasma amino acid levels of the essential amino acids were not different between two groups ( Figure 3E). Thus, the concentrations of the branched chain amino acids (BCAA), Tyr, and Phe, which were previously implicated in heart failure, a form of CVD [51][52][53], were similar in both the HF-fed and control groups. As shown in Table A1, both diets contained the same amount of casein, which was the only protein source. Because essential amino acids cannot be synthetized endogenously, this observation suggests that the metabolic pathways responsible for utilization and catabolism of essential amino acids are intact in the HF-fed animals. Among the remaining amino acids, significantly lower levels of alanine (Ala), asparagine (Asn), lysine (Lys), serine (Ser), and proline (Pro) were found in the plasma of the HF-fed mice compared to the control group ( Figure 3E), suggesting that the HF diet resulted in a decreased protein turn-over state that is consistent with preferential utilization of the dietary fat as energy fuel [54].

Systemic Inflammation
Finally, we determined the effect of the diets on plasma proinflammatory cytokines, including interferon gamma (IFN-γ), interleukin 6 (IL-6), macrophage inflammatory protein-1 alpha (MIP-1α/CCL3), and tumor necrosis factor alpha (TNF-α) [42,55,56]. IFN-γ is a proinflammatory mediator that is expressed in atherosclerotic lesions [57]. IL-6 is a pleiotropic cytokine with both pro-or antiatherogenic effects but which exacerbates atherosclerosis in murine species [58,59] MCP-1 is an inflammatory chemokine with a critical role in the initiation of atherosclerosis [60]. TNF-α is another major proatherogenic molecule that sustains the progression of the vascular lesions and atherosclerosis [42]. As expected, feeding mice with HF diet significantly elevated the systemic concentration of most cytokines, when compared to the control diet-fed mice (Figure 4). This observation is in agreement with the positive effect of dietary fat on systemic inflammation and with the well-established atherogenic effect of a HF diet in apoE −/− mice [61,62]. In fact, inflammation is central to all stages of atherosclerosis establishment and progression.

Volume of Atherosclerosis
A method based on ex-vivo MR imaging was used to visualize and quantify the aortic volume of fatty plaque in HF-fed mice versus controls. The results revealed a profound difference of the atherosclerosis burden between the two groups of animals ( Figure 5). Plaque volume throughout the whole aorta was significantly increased in the HF group compared to the control group; the atherosclerotic lesions predominantly distributed in the aortic arch and in the areas surrounding the branching points of the major arteries, which includes the brachiocephalic artery (BCA). As a result, the difference between HFaortas and control aortas in these two highly susceptible regions, aortic arch and BCA, was even more pronounced than in the whole aortas. Interestingly, despite this massive difference of vascular lesions between HF-fed mice and controls both groups of animals presented similar plasma concentrations of the BCAA Val, Leu and Ile, and of Tyr and Phe (Figure 3), which were previously suggested to have a significant role in the pathogenesis of atherosclerosis and CVD [51][52][53]. The increased systemic inflammation observed in HF-fed mice, however, (Figure 4) is consistent with this augmented arteriosclerotic plaque burden observed in these mice. Taken together, these observations are in agreement with the well-established atherogenic effect of dietary fat [63]. Having induced a strong atherosclerotic phenotype with HF diet in apoE −/− mice, when compared to controls, we determined the expression levels of the orthodox AQP isoforms, AQP1 and AQP5, and of the aquaglyceroporins AQP3, AQP7, and AQP9, in both groups of mice.
cule that sustains the progression of the vascular lesions and atherosclerosis [42]. As expected, feeding mice with HF diet significantly elevated the systemic concentration of most cytokines, when compared to the control diet-fed mice (Figure 4). This observation is in agreement with the positive effect of dietary fat on systemic inflammation and with the well-established atherogenic effect of a HF diet in apoE -/-mice [61,62]. In fact, inflammation is central to all stages of atherosclerosis establishment and progression.

Volume of Atherosclerosis
A method based on ex-vivo MR imaging was used to visualize and quantify the aortic volume of fatty plaque in HF-fed mice versus controls. The results revealed a profound difference of the atherosclerosis burden between the two groups of animals ( Figure 5). Plaque volume throughout the whole aorta was significantly increased in the HF group compared to the control group; the atherosclerotic lesions predominantly distributed in the aortic arch and in the areas surrounding the branching points of the major arteries, which includes the brachiocephalic artery (BCA). As a result, the difference between HFaortas and control aortas in these two highly susceptible regions, aortic arch and BCA, was even more pronounced than in the whole aortas. Interestingly, despite this massive

Expression of Aquaporins in Liver and Adipose Tissues
The expression of AQPs with impact on metabolism and endothelial function (AQP1, AQP3, AQP5, AQP7, and AQP9) [14] was evaluated in three distinct metabolic tissues: liver, WAT and BAT. Liver is the organ responsible for the plasma glucose levels maintenance, thus, in a situation of negative energy balance or exercise, glucose is produced in the liver and released to the bloodstream [64]. When the body is in a state of positive energy balance and plasma glucose levels are high (as in the HF-fed mice), energy is stored in WAT in the triacylglycerol form, to be hydrolyzed in case of energy demands. BAT is a tissue that burns excess fat by thermogenesis [65].
All of the investigated AQP isoforms were detected in liver, WAT, and BAT, however, each in a tissue-specific profile ( Figure 6A,C,E, black bars). In liver, and as previously described, AQP9 was the most highly expressed isoform [18]. Moreover, AQP1, AQP3, AQP7, and AQP5 were also detected in liver, although at lower levels than AQP9 ( Figure  6A). While AQP9 has been described as responsible for glycerol influx in hepatocytes, no specific function has been attributed to any other hepatic AQP isoform [66]. Concerning the impact of the HF diet on the hepatic AQPs expression levels, we observed that AQP3 was the most affected isoform, with the HF-fed mice presenting levels that were around 80-fold more than controls. Nevertheless, since the overall expression level of AQP3 was lower than the most abundant isoform AQP9, an increase in AQP3 expression may not have significantly impacted the total glycerol efflux in hepatocytes. Interestingly, however, AQP3 is also a hydrogen peroxide channel [67], and thus its upregulation might represent an increase in oxidative stress in the tissue. This possibility is favored by the fact that the hepatic expression levels of another peroxiporin, AQP5, were also significantly upregulated by the atherogenic diet. In fact, overall oxidative stress is a major driver of the strong atherogenic phenotype observed in these HF-fed animals [46,48]. Additionally, the results showed that hepatic AQP1 was significantly downregulated by the HF-diet whereas the levels of AQP9 remained unaltered ( Figure 6B). One limitation to our study is the fact that AQP9 immunolocalization was not performed, which would show whether it is correlated with gene expression or liver pathology. By histopathological analysis we could confirm the presence of hepatic fat accumulation in the HF-fed mice. Nevertheless, the lack of markers of hepatic fibrosis in the present study prevents us to further elucidate the relationship between hepatic AQPs expression and the development of atherosclerosis. However, a study in patients with morbid obesity did not find any relationship between AQP9 expression in the liver and the degree of hepatic steatosis or fibrosis [68]. Therefore, it seems that regulation of fatty liver deposits is not influenced by AQP9 expression. Thus, it is unlikely that animals fed the atherogenic diet and with liver fat deposition show altered hepatic aquaglyceroporin expression profile.
Biomedicines 2021, 9, x FOR PEER REVIEW 10 of 16 difference of vascular lesions between HF-fed mice and controls both groups of animals presented similar plasma concentrations of the BCAA Val, Leu and Ile, and of Tyr and Phe (Figure 3), which were previously suggested to have a significant role in the pathogenesis of atherosclerosis and CVD [51][52][53]. The increased systemic inflammation observed in HF-fed mice, however, (Figure 4) is consistent with this augmented arteriosclerotic plaque burden observed in these mice. Taken together, these observations are in agreement with the well-established atherogenic effect of dietary fat [63]. Having induced a strong atherosclerotic phenotype with HF diet in apoE −/− mice, when compared to controls, we determined the expression levels of the orthodox AQP isoforms, AQP1 and AQP5, and of the aquaglyceroporins AQP3, AQP7, and AQP9, in both groups of mice.

Expression of Aquaporins in Liver and Adipose Tissues
The expression of AQPs with impact on metabolism and endothelial function (AQP1, AQP3, AQP5, AQP7, and AQP9) [14] was evaluated in three distinct metabolic tissues: liver, WAT and BAT. Liver is the organ responsible for the plasma glucose levels maintenance, thus, in a situation of negative energy balance or exercise, glucose is produced in the liver and released to the bloodstream [64]. When the body is in a state of positive en-  The downregulation of AQP3 in parallel with the dramatic upregulation of AQP5, suggests an impairment of glycerol movements coupled to increased sensitivity to oxidative stress, similar to the observed in WAT. The AQPs expression pattern in the three analyzed tissues revealed tissue-specific differences, inherent to their predicted metabolic role. However, AQP5 expression was consistently induced by HF diet across WAT, BAT, and liver. Since AQP5 facilitates hydrogen peroxide permeation in rodents and humans, its upregulation suggests the presence of an unbalanced redox state [13,69]. Here, we have detected similar plasma levels of total glutathione, the major intracellular antioxidant that neutralizes hydrogen peroxide, in both groups of animals, thereby suggesting that the bioavailability of glutathione was intact in the HF-fed mice. Nevertheless, this similar glutathione bioavailability does not translate into a similar antioxidant capacity and could only be evaluated by determining Interestingly, the association of an inadequate AQP1 function with an atherogenic phenotype has been previously reported albeit in other contexts. For example, we observed that endothelial dysfunction under hypomethylating stress lessened AQP1 expression in vitro [14], and others reported that the targeted deletion of the AQP1 gene favored atherosclerosis progression in apoE −/− mice [4]. In the present study, and as discussed above, the AdoMet/AdoHcy ratio was not affected by the HF-diet, so we can exclude a disturbed systemic methylation index as causing the observed HF-induced hepatic AQP1 downregulation.
In WAT, we have detected abundant levels of AQP1, AQP3 and AQP7 mRNAs, while AQP5 and AQP9 were present in low amounts ( Figure 6C) [16,66]. A strong induction of AQP5 expression of around 15-fold was observed in mice fed HF diet. As aforementioned, AQP5 is a peroxiporin whose expression has been related with oxidative stress in rodents [69] and humans [13]. We have previously reported dysregulation of endothelial AQP5 expression associated with endothelial dysfunction [14], suggesting AQP5 is closely related to a dysbalanced redox state that favors the atherosclerosis progression. In addition, our present results show that AQP3 and AQP9 expression is impaired in WAT from mice fed HF diet ( Figure 6D). Knowing that AQP3 and AQP9 are the most abundant aquaglyceroporins in mice adipose membranes [26], their downregulation might represent a cellular strategy to avoid excess glycerol efflux to be used in liver.
BAT presented high levels of AQP7 gene expression, the most representative isoform in this tissue, and low levels of AQP1, AQP3, AQP5, and AQP9 mRNAs ( Figure 6E). In the BAT of HF-fed mice, AQP3 was downregulated, AQP9 and AQP5 were increased around 4-and 60-fold, respectively, while AQP1 and AQP7 expression was unaltered ( Figure 6F). The downregulation of AQP3 in parallel with the dramatic upregulation of AQP5, suggests an impairment of glycerol movements coupled to increased sensitivity to oxidative stress, similar to the observed in WAT.
The AQPs expression pattern in the three analyzed tissues revealed tissue-specific differences, inherent to their predicted metabolic role. However, AQP5 expression was consistently induced by HF diet across WAT, BAT, and liver. Since AQP5 facilitates hydrogen peroxide permeation in rodents and humans, its upregulation suggests the presence of an unbalanced redox state [13,69]. Here, we have detected similar plasma levels of total glutathione, the major intracellular antioxidant that neutralizes hydrogen peroxide, in both groups of animals, thereby suggesting that the bioavailability of glutathione was intact in the HF-fed mice. Nevertheless, this similar glutathione bioavailability does not translate into a similar antioxidant capacity and could only be evaluated by determining the concentration of the reduced and oxidized glutathione forms. AQP5 dysfunction has been associated with a vast array of phenotypes, and evidence suggests that AQP5 upregulation promotes tumor cell proliferation [70,71]. Studies correlating AQP5 to obesity are scarce; however, a link between hypothalamus AQP5 and adipocyte gene expression was reported, indicating a possible regulatory coordination [72]. Interestingly, AQP5 is crucial for mice adipocyte differentiation [73] and AQP5-KO mice have lower body weight than controls [74]. Altogether, these data suggest that the increased AQP5 expression observed in WAT may indicate an increase in adipocyte differentiation to accommodate the excess fat in HF diet.

Conclusions
In conclusion, the present study contributes to a better characterization of the wellestablished apoE −/− mouse model by reporting that the pattern of AQPs expression in these mice is disturbed, in a tissue-specific manner, by an atherogenic HF diet. The present report suggests a novel relation between diet, AQP5, and atherosclerosis that warrants further investigation and may ultimately open the door to the development of effective new treatments for CVD.