Summary
Cells isolated from pig urinary bladders and pregnant full term human uteruses were attached longitudinally between a microforce transducer and a length displacement apparatus. Cells were stretched by applying a series of ramp-like length changes of 0.2 s duration and 10.0μm amplitude at intervals of 15 min. Passive forces upon straining were as high as 70–100μN. Following these peak forces stress relaxation occurred, levelling off approximately 50% of the maximum peak force. The maximum elastic modulus estimated for single cells was found to be at least a tenfold higher than was previously estimated from intact bladder strips. The relation between the increase in length and the increase in initial force increment was found to be approximately linear. An exponential equation was fitted to a selected number of stress relaxation curves. Relaxation curves of bladder cells show a clearly different time course as compared to bladder tissue strips, suggesting that a significant amount of relaxation in strips has to be contributed to the connective tissue components or to structural changes in these strips.
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References
Alexander, R. S. (1957) Elasticity of muscular organs. In:Tissue Elasticity (edited byRemington, J. W.). Washington DC: American Physiology Society.
Coolsaet, B. L. R. A. (1977) Stepwise cystometry. A new method to investigate properties of the urinary bladder. PhD Thesis. Rotterdam: Erasmus University, Rotterdam.
Coolsaet, B. L. R. A., Duyl, W. A. Van, Mastrigt, R. Van &Schouten, J. W. (1975a) Visco-elastic properties of bladder wall strips.Inv. Urol. 12, 351–6.
Coolsaet, B. L. R. A., Duyl, W. A. Van, Mastrigt, R. Van &Zwart, A. Van Der (1975b) Visco-elastic properties of the bladder wall.Urol. Int. 30, 16–26.
Dijk, A. M. Van, Wieringa, P. A., Meer, M. Van Der &Laird, J. D. (1984) Mechanics of resting isolated single vascular smooth muscle cells from bovine coronary artery.Am. J. Physiol. 246, C277–87.
Douglas, W. R. (1972) Of pigs and men and research.Space Life Sci. 3, 226–34.
Drogendijk, A. C., Duyl, W. A. Van &Zon, A. T. M. Van Der (1988) Provocation of contraction pikes of the cervix uteri by dilatation: Diagnostic significance for premature labour. In Proceedings of XII World Congress of Gynecology and Obstetrics, Rio de Janeiro, Brazil, October 23–28.
Duyl, W. A. Van, Zon, A. T. M. Van Der, Oomens, C. W. J. &Drogendijk, A. C. (1987) Stress relaxation, used as a tool for diagnosis of incompetence of human cervix in terms of a mixture model of tissue. In:Biomechanics: Basic and Applied Research (edited byBergman, G., Kolbel, R. &Rohlmann, A.) pp. 193–8. Amsterdam: Martinus Nijhoff Publications.
Fay, F. S. (1975) Mechanical properties of single isolated smooth muscle cellsINSERM,50, 327–42.
Fung, Y. G. B. (1967) Elasticity of soft tissues in simple elongation.Am. J. Physiol. 213, 1532–44.
Fung, Y. C. (1981)Biomechanics: Mechanical Properties of Living Tissues. Berlin and New York: Springer-Verlag.
Glerum, J. J. &Mastrigt, R. van (1990a) Mechanical properties of mammalian single smooth muscle cells, part I: A low cost large range microforce transducer.J. Musc. Res. Cell Motil. 11, 331–7.
Glerum, J. J. &Mastrigt, R. Van (1990b) Mechanical properties of mammalian single smooth muscle cells, part II: Evaluation of a modified technique for attachment cells to the measurement apparatus.J. Musc. Res. Cell Motil 11, 338–43.
Glerum, J. J., Mastrigt, R. Van, Romijn, J. C. &Griffiths, D. J. (1987) Isolation and individual electrical stimulation of single smooth-muscle cells from the urinary bladder of the pig.J. Musc. Res. Cell Mot. 8, 125–34.
Gosling, J. A., Dixon, J. S. &Humpherson, J. R. (1983)Functional Anatomy of the Urinary Tract. pp. 3.16–18. London and New York: Gower Medical Publishing.
Hai, C. M. &Murphy, R. A. (1988a) Cross-bridge phosphorylation and regulation of latch state in smooth muscle.Am. J. Physiol. 254, C99–106.
Hai, C. M. &Murphy, R. A. (1988b) Regulation of shortening velocity by cross-bridge phosphorylation in smooth muscle.Am. J. Physiol. 255, C86–94.
Ham, A. W. (1974)Histology. 7th edition, pp 869–70. Philadelphia and Toronto: J. B. Lippincott Company.
Kirkegaard, D. (1970)A Fortran IV Version of the Sum-of-Exponential Least Squares Code Exposum. Riso: Danish Atomic Energy Commission, Research Establishment.
Kondo, A. &Susset, J. G. (1972) Cystometrie rapide-principe et application clinique.L'union Med. du Can. 101, 1141–5.
Levin, R. M., Ruggieri, M. R., Velagapudi, S., Gordon, D., Altman, B. &Wein, A. J. (1986) Relevance of spontaneous activity to urinary bladder function: anin vitro andin vivo study.J. Urol. 136, 517–21.
Marston, S. B. (1989) What is latch? New ideas about tonic contraction in smooth muscle.J. Musc. Res. Cell Mot. 10, 97–100.
Ray, C. D. (1974)Medical Engineering. pp. 199–215, 558–66. Chicago: Year Book Publishers.
Regnier, C. H., Meyer, S., Susset, J. G. &Elbadawi, A. (1989) Biomechanical/microstructural basis of bladder compliance: A model proposal, and a preliminary experimental study. InProceedings of the Urodynamics Society, Texas, USA, May6, 1989.
Remington, J. W. (1957) Extensibility behaviour and hystereris phenomena in smooth muscle tissues. InTissue Elasticity (edited byRemington, J. W.). Washington DC: American Physiology Society.
Small, J. V., FÜrst, D. O. &Mey, J. de (1986) Localization of filamin in smooth muscle.J. Cell Biol. 102, 210–20.
Squire, J. (1981)The Structural Basis of Muscular Contraction. pp. 443–61. New York and London: Plenum Press.
Stephens, N. L. (1984)Smooth Muscle Contraction. New York and Basel: Marcel Dekker Inc.
Susset, J. G. &Regnier, C. H. (1981) Viscoelastic properties of bladder strips. Standardization of a technique.Inv. Urol. 18, 445–50.
Van Mastrigt, R. (1977) A systems approach to the passive properties of the urinary bladder in the collection phase. PhD Thesis, Erasmus University, Rotterdam, pp 80–95. Delft: Delft University Press.
Van Mastrigt, R. (1988) The length dependence of the series elasticity of pig bladder smooth muscle.J. Musc. Res. Cell Mot. 9, 525–32.
VanMastrigt, R. (1989) The force recovery following repeated quick releases applied to pig urinary bladder smooth muscle.J. Muse. Res. Cell Mot., submitted for publication.
Van Mastrigt, R., Coolsaet, B. L. R. A. &Duyl, W. A. Van (1978) Passive properties of the urinary bladder in the collection phase.Med. & Biol. Eng. & Comput. 16, 471–82.
Van Mastrigt, R., Coolsaet, B. L. R. A. &Duyl, W. A. Van (1981) First results of stepwise straining of the human urinary bladder and human bladder strips.Inv. Urol. 19, 58–61.
Zon, A. T. M. Van Der, Duyl, W. A. Van &Drogendijk A. C. (1989) Measurement of cervical relaxation: A new diagnostic tool for cervical incompetence.Obstet. and Gynec., submitted for publication.
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Glerum, J.J., Van Mastrigt, R. & Van Koeveringe, A.J. Mechanical properties of mammalian single smooth muscle cells III. Passive properties of pig detrusor and human a terme uterus cells. J Muscle Res Cell Motil 11, 453–462 (1990). https://doi.org/10.1007/BF01739765
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DOI: https://doi.org/10.1007/BF01739765