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Mechanical properties of mammalian single smooth muscle cells I. A low cost large range microforce transducer

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Summary

A transducer has been developed for measuring the minute forces generated during isometric contractions (1.0–10.0μN) of single smooth muscle cells from the pig urinary bladder and the human uterus. In addition to its high sensitivity, resolution and stability (100 mVμN−1, <0.1μN and <2.0μN h−1), the transducer features a very wide range (100–140μN) with good linearity, enabling measurement of contractions as well as passive force-length characteristics within one uninterrupted measurement session. Since the transducer features an independent and interchangeable force to displacement conversion system, different force ranges can be realized by inserting force conversion systems with different compliances.

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References

  • Brehm, R. &Grootel, P. Van (1985) Toepassing van glaskeramiek in een precisie-meetapparaat.Nederlandse Vereniging voor Glastechniek informatie,2, 2–3.

    Google Scholar 

  • Canaday, P.G. &Fay, F. S. (1976) An ultrasensitive isometric force transducer for single smooth muscle cell mechanics.J. Appl. Physiol. 40, 243–6.

    PubMed  Google Scholar 

  • Dijk, A. M. Van, Wieringa, P. A., Meer, M. Van Der &Laird, J. D. (1984) Mechanics of resting isolated single vascular smooth muscle cells from bovine coronairy artery.Am. J. Physiol. 246, C277–87.

    PubMed  Google Scholar 

  • Glerum, J. J., Mastrigt, R. Van, (1990) Mechanical properties of mammalian single smooth muscle cells, II: Evaluation of a modified technique for attachment of cells to the measurement apparatus.J. Musc. Res. Cell Motil. 11, 338–343.

    Google Scholar 

  • Ishii, N. &Takahashi, K. (1982) Length-tension relation of single smooth muscle cells isolated from the pedal retractor muscle ofMytilus edulis.J. Musc. Res. Cell Mot. 3, 25–38.

    Google Scholar 

  • Ishii, N., Simpson, A. W. M. &Ashley, C. C. (1988) Intracellular free calcium ([Ca2+]i) and the ‘catch’ contraction in isolated molluscan smooth-muscle (ABRM) cells. (abstract)J. Musc. Res. Cell Mot. 9, 463.

    Google Scholar 

  • Iwazumi, T. (1982) High performance instrument for myofibrillar mechanics, (abstract)Bioph. J. 37, 357a.

    Google Scholar 

  • McLaughlin, R. J. (1977) Systematic design of cantilever beams for muscle research.J. Appl. Physiol. 42, 786–94.

    PubMed  Google Scholar 

  • Meiss, R. A. (1971) An isometric muscle force transducer.J. Appl. Physiol. 30, 158–60.

    PubMed  Google Scholar 

  • Meiss, R. A. (1974) A versatile transducer system for mechanical studies of muscle.J. Appl. Physiol. 37, 459–63.

    PubMed  Google Scholar 

  • Minns, H. G. (1971) A voltage-controlled force generator for calibrating sensitive transducers.J. Appl. Physiol. 30, 895–6.

    PubMed  Google Scholar 

  • Minns, H. G. &Franz, G. N. (1972) A low-drift transducer for small forces.J. Appl Physiol 33, 529–31.

    PubMed  Google Scholar 

  • Tung, L. (1986) An ultrasensitive transducer for measurement of isometric contractile force from single heart cells.Pflügers Arch. 407, 109–15.

    Google Scholar 

  • Warshaw, D. M. &Fay, F. S. (1983) Cross-bridge elasticity in single smooth muscle cells.J. Gen. Physiol. 82, 157–99.

    PubMed  Google Scholar 

  • Wieringa, P. A., Meer, M. Van Der, Dijk, A. M. Van &Laird, J. D. (1984) Sensitive force transducer system for mechanical studies of single isolated vascular smooth muscle cells.Med. & Biol. Eng. & Comp. 22, 130–7.

    Google Scholar 

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Authors and Affiliations

  1. Department of Urology, Erasmus University, Room EE 1630, P.O. Box 1738, 3000, DR Rotterdam, The Netherlands

    J. J. Glerum & R. Van Mastrigt

Authors
  1. J. J. Glerum
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  2. R. Van Mastrigt
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Glerum, J.J., Van Mastrigt, R. Mechanical properties of mammalian single smooth muscle cells I. A low cost large range microforce transducer. J Muscle Res Cell Motil 11, 331–337 (1990). https://doi.org/10.1007/BF01766671

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  • DOI: https://doi.org/10.1007/BF01766671

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