NoteComparison of the DiversiLab™ system, Pulsed-Field Gel Electrophoresis and Multi-Locus Sequence Typing for the characterization of epidemic reference MRSA strains
Section snippets
Acknowledgements
The DiversiLab™ kits for S. aureus characterization used in this study were supplied free of charge by bioMérieux. There is no conflict of interests.
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2015, Diagnostic Microbiology and Infectious DiseaseCitation Excerpt :Performing PFGE on representative isolates within a DL rep-PCR cluster may not be sufficient for classification. Mixed PFGE types within 1 DL cluster have been reported for several PFGE types such as USA300/500, USA100/800, USA 400/700/800, CMRSA-7/8, CMRSA-10/9/5, and CMRSA-3/6 (Church et al., 2011; Tenover et al., 2009; Te Witt et al., 2009). For USA300 and 500, differentiation is apparent on the pattern overlay function; a unique peak is present at the 660 data point that is not present for USA300 (Tenover et al., 2009).
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2013, Journal of Microbiological MethodsCitation Excerpt :These spa types were not mentioned in the previous Australian study (Stephens, et al., 2008). The reagent cost of the HRM method was at 6 USD per patient samples including the duplicate run, which was less than one-fifth of the conventional methods (Te Witt et al., 2009). The turnaround time for this HRM-based real-time PCR could be shortened to 3 h.
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2011, Journal of Microbiological MethodsCitation Excerpt :Conversely, the polymorphism observed by MLST within certain rPCs (Fig. 1) might reflect exchange of large chromosomal segments between strains belonging to different STs, as demonstrated under laboratory conditions (Brochet et al., 2008). These results are slightly different from those previously reported for typing of other Gram positive cocci, such as S. aureus (Te Witt et al., 2009), and Gram negative bacteria, such as E. coli (Bonacorsi et al., 2009), that demonstrated a good agreement between DL and MLST. It is worth mentioning that the strains examined in these studies were S. aureus methicillin-resistant and E. coli neonatal-meningitis strains, two populations displaying low level of genetic diversity and thus favouring a good correlation between these 2 methods (Bonacorsi and Bingen, 2005; Lindsay, 2010).