Note
Comparison of the DiversiLab™ system, Pulsed-Field Gel Electrophoresis and Multi-Locus Sequence Typing for the characterization of epidemic reference MRSA strains

https://doi.org/10.1016/j.mimet.2009.01.009Get rights and content

Abstract

We have analyzed a representative selection of the HARMONY meticillin-resistant Staphylococcus aureus strain collection originating from 11 European countries (Cookson, B.D. et al., 2007, J. Clin. Microbiol. 45: 1830–1837) with the DiversiLab™ System, Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing (MLST). Simpson's diversity indices were 0.905, 0.877 and 0.860 for PFGE, MLST and DiversiLab™, respectively. All methods displayed concordant classification of the MRSA strains, although with divergent resolution and reproducibility.

Section snippets

Acknowledgements

The DiversiLab™ kits for S. aureus characterization used in this study were supplied free of charge by bioMérieux. There is no conflict of interests.

References (21)

  • EnrightM.C. et al.

    Multilocus sequence typing

    Trends in Microbiology

    (1999)
  • MaquelinK. et al.

    Current trends in the epidemiological typing of clinically relevant microbes in Europe

    Journal of Microbiological Methods

    (2007)
  • SternM.J. et al.

    Repetitive extragenic palindromic sequences: a major component of the bacterial genome

    Cell

    (1984)
  • AbramsonM.A. et al.

    Nosocomial methicillin-resistant and methicillin-susceptible Staphylococcus aureus primary bacteremia: at what costs?

    Infection Control and Hospital Epidemiology

    (1999)
  • Bart-DelabesseE. et al.

    Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates

    Journal of Clinical Microbiology

    (2001)
  • CooksonB.D. et al.

    Evaluation of molecular typing methods in characterizing a European collection of epidemic methicillin-resistant Staphylococcus aureus strains: the HARMONY collection

    Journal of Clinical Microbiology

    (2007)
  • CosgroveS.E. et al.

    Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis

    Clinical Infectious Diseases

    (2003)
  • DeplanoA. et al.

    Multicenter evaluation of epidemiological typing of methicillin-resistant Staphylococcus aureus strains by repetitive-element PCR analysis. The European Study Group on Epidemiological Markers of the ESCMID

    Journal of Clinical Microbiology

    (2000)
  • EnrightM.C. et al.

    The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA)

    Proceedings of the National Academy of Sciences of the United States of America

    (2002)
  • HealyM. et al.

    Microbial DNA typing by automated repetitive-sequence-based PCR

    Journal of Clinical Microbiology

    (2005)
There are more references available in the full text version of this article.

Cited by (33)

  • Evaluation of repetitive element polymerase chain reaction for surveillance of methicillin-resistant Staphylococcus aureus at a large academic medical center and community hospitals

    2015, Diagnostic Microbiology and Infectious Disease
    Citation Excerpt :

    Performing PFGE on representative isolates within a DL rep-PCR cluster may not be sufficient for classification. Mixed PFGE types within 1 DL cluster have been reported for several PFGE types such as USA300/500, USA100/800, USA 400/700/800, CMRSA-7/8, CMRSA-10/9/5, and CMRSA-3/6 (Church et al., 2011; Tenover et al., 2009; Te Witt et al., 2009). For USA300 and 500, differentiation is apparent on the pattern overlay function; a unique peak is present at the 660 data point that is not present for USA300 (Tenover et al., 2009).

  • A Phylogenetic Perspective on Molecular Epidemiology

    2014, Molecular Medical Microbiology
  • The use of high-resolution melting analysis for rapid spa typing on methicillin-resistant Staphylococcus aureus clinical isolates

    2013, Journal of Microbiological Methods
    Citation Excerpt :

    These spa types were not mentioned in the previous Australian study (Stephens, et al., 2008). The reagent cost of the HRM method was at 6 USD per patient samples including the duplicate run, which was less than one-fifth of the conventional methods (Te Witt et al., 2009). The turnaround time for this HRM-based real-time PCR could be shortened to 3 h.

  • Comparison of the Diversilab® system with multi-locus sequence typing and pulsed-field gel electrophoresis for the characterization of Streptococcus agalactiae invasive strains

    2011, Journal of Microbiological Methods
    Citation Excerpt :

    Conversely, the polymorphism observed by MLST within certain rPCs (Fig. 1) might reflect exchange of large chromosomal segments between strains belonging to different STs, as demonstrated under laboratory conditions (Brochet et al., 2008). These results are slightly different from those previously reported for typing of other Gram positive cocci, such as S. aureus (Te Witt et al., 2009), and Gram negative bacteria, such as E. coli (Bonacorsi et al., 2009), that demonstrated a good agreement between DL and MLST. It is worth mentioning that the strains examined in these studies were S. aureus methicillin-resistant and E. coli neonatal-meningitis strains, two populations displaying low level of genetic diversity and thus favouring a good correlation between these 2 methods (Bonacorsi and Bingen, 2005; Lindsay, 2010).

View all citing articles on Scopus
View full text