1887

Abstract

The threat of emerging infectious viruses in humans requires a more effective approach regarding virus surveillance. A thorough understanding of virus diversity in wildlife provides epidemiological baseline information about pathogens and may lead to the identification of newly emerging pathogens in the future. In this study, diarrhoea samples from an outbreak of gastrointestinal illness in a Danish population of European roe deer were gathered for which no aetiological agent could be identified. Large-scale molecular RNA virus screening, based on host nucleic acid depletion, sequence-independent amplification and sequencing of partially purified viral RNA, revealed the presence of novel astroviruses, CcAstV-1 and CcAstV-2, in two of ten diarrhoea samples. Whether these viruses were responsible for causing diarrhoea remains to be determined. Phylogenetic analyses on amplified sequences showed that these viruses were most closely related to each other, were a novel species in the genus and may represent two different serotypes.

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2010-11-01
2024-03-28
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References

  1. Akihara, S., Phan, T. G., Nguyen, T. A., Hansman, G., Okitsu, S. & Ushijima, H.(2005). Existence of multiple outbreaks of viral gastroenteritis among infants in a day care center in Japan. Arch Virol 150, 2061–2075.[CrossRef] [Google Scholar]
  2. Allander, T., Emerson, S. U., Engle, R. E., Purcell, R. H. & Bukh, J.(2001). A virus discovery method incorporating DNase treatment and its application to the identification of two bovine parvovirus species. Proc Natl Acad Sci U S A 98, 11609–11614.[CrossRef] [Google Scholar]
  3. Allander, T., Tammi, M. T., Eriksson, M., Bjerkner, A., Tiveljung-Lindell, A. & Andersson, B.(2005). Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A 102, 12891–12896.[CrossRef] [Google Scholar]
  4. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  5. Atkins, A., Wellehan, J. F., Jr, Childress, A. L., Archer, L. L., Fraser, W. A. & Citino, S. B.(2009). Characterization of an outbreak of astroviral diarrhea in a group of cheetahs (Acinonyx jubatus). Vet Microbiol 136, 160–165.[CrossRef] [Google Scholar]
  6. Finkbeiner, S. R., Kirkwood, C. D. & Wang, D.(2008). Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea. Virol J 5, 117.[CrossRef] [Google Scholar]
  7. Finkbeiner, S. R., Holtz, L. R., Jiang, Y., Rajendran, P., Franz, C. J., Zhao, G., Kang, G. & Wang, D.(2009a). Human stool contains a previously unrecognized diversity of novel astroviruses. Virol J 6, 161.[CrossRef] [Google Scholar]
  8. Finkbeiner, S. R., Le, B. M., Holtz, L. R., Storch, G. A. & Wang, D.(2009b). Detection of newly described astrovirus MLB1 in stool samples from children. Emerg Infect Dis 15, 441–444.[CrossRef] [Google Scholar]
  9. Finkbeiner, S. R., Li, Y., Ruone, S., Conrardy, C., Gregoricus, N., Toney, D., Virgin, H. W., Anderson, L. J., Vinje, J. & other authors(2009c). Identification of a novel astrovirus (astrovirus VA1) associated with an outbreak of acute gastroenteritis. J Virol 83, 10836–10839.[CrossRef] [Google Scholar]
  10. Glass, R. I., Noel, J., Mitchell, D., Herrmann, J. E., Blacklow, N. R., Pickering, L. K., Dennehy, P., Ruiz-Palacios, G., de Guerrero, M. L. & Monroe, S. S.(1996). The changing epidemiology of astrovirus-associated gastroenteritis: a review. Arch Virol Suppl 12, 287–300. [Google Scholar]
  11. Gough, R. E., Collins, M. S., Borland, E. & Keymer, L. F.(1984). Astrovirus-like particles associated with hepatitis in ducklings. Vet Rec 114, 279. [Google Scholar]
  12. Imada, T., Yamaguchi, S., Mase, M., Tsukamoto, K., Kubo, M. & Morooka, A.(2000). Avian nephritis virus (ANV) as a new member of the family Astroviridae and construction of infectious ANV cDNA. J Virol 74, 8487–8493.[CrossRef] [Google Scholar]
  13. Indik, S., Valicek, L., Smid, B., Dvorakova, H. & Rodak, L.(2006). Isolation and partial characterization of a novel porcine astrovirus. Vet Microbiol 117, 276–283.[CrossRef] [Google Scholar]
  14. Kapoor, A., Li, L., Victoria, J., Oderinde, B., Mason, C., Pandey, P., Zaidi, S. Z. & Delwart, E.(2009). Multiple novel astrovirus species in human stool. J Gen Virol 90, 2965–2972.[CrossRef] [Google Scholar]
  15. Klein, E. J., Boster, D. R., Stapp, J. R., Wells, J. G., Qin, X., Clausen, C. R., Swerdlow, D. L., Braden, C. R. & Tarr, P. I.(2006). Diarrhea etiology in a Children's Hospital Emergency Department: a prospective cohort study. Clin Infect Dis 43, 807–813.[CrossRef] [Google Scholar]
  16. Kuiken, T., Leighton, F. A., Fouchier, R. A., LeDuc, J. W., Peiris, J. S., Schudel, A., Stohr, K. & Osterhaus, A. D.(2005). Public health. Pathogen surveillance in animals. Science 309, 1680–1681.[CrossRef] [Google Scholar]
  17. Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A. & other authors(2007).clustalw and clustal_x version 2.0. Bioinformatics 23, 2947–2948.[CrossRef] [Google Scholar]
  18. Lukashov, V. V. & Goudsmit, J.(2002). Evolutionary relationships among Astroviridae. J Gen Virol 83, 1397–1405. [Google Scholar]
  19. Lyman, W. H., Walsh, J. F., Kotch, J. B., Weber, D. J., Gunn, E. & Vinje, J.(2009). Prospective study of etiologic agents of acute gastroenteritis outbreaks in child care centers. J Pediatr 154, 253–257.[CrossRef] [Google Scholar]
  20. Mittelholzer, C., Englund, L., Hedlund, K. O., Dietz, H. H. & Svensson, L.(2003). Detection and sequence analysis of Danish and Swedish strains of mink astrovirus. J Clin Microbiol 41, 5192–5194.[CrossRef] [Google Scholar]
  21. Moser, L. A. & Schultz-Cherry, S.(2005). Pathogenesis of astrovirus infection. Viral Immunol 18, 4–10.[CrossRef] [Google Scholar]
  22. Rivera, R., Nollens, H. H., Venn-Watson, S., Gulland, F. M. & Wellehan, J. F., Jr(2010). Characterization of phylogenetically diverse astroviruses of marine mammals. J Gen Virol 91, 166–173.[CrossRef] [Google Scholar]
  23. Soares, C. C., Maciel de Albuquerque, M. C., Maranhao, A. G., Rocha, L. N., Ramirez, M. L., Benati, F. J., Timenetsky Mdo, C. & Santos, N.(2008). Astrovirus detection in sporadic cases of diarrhea among hospitalized and non-hospitalized children in Rio De Janeiro, Brazil, from 1998 to 2004. J Med Virol 80, 113–117.[CrossRef] [Google Scholar]
  24. Strain, E., Kelley, L. A., Schultz-Cherry, S., Muse, S. V. & Koci, M. D.(2008). Genomic analysis of closely related astroviruses. J Virol 82, 5099–5103.[CrossRef] [Google Scholar]
  25. Svraka, S., Duizer, E., Vennema, H., de Bruin, E., van der Veer, B., Dorresteijn, B. & Koopmans, M.(2007). Etiological role of viruses in outbreaks of acute gastroenteritis in The Netherlands from 1994 through 2005. J Clin Microbiol 45, 1389–1394.[CrossRef] [Google Scholar]
  26. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  27. Toffan, A., Jonassen, C. M., De Battisti, C., Schiavon, E., Kofstad, T., Capua, I. & Cattoli, G.(2009). Genetic characterization of a new astrovirus detected in dogs suffering from diarrhoea. Vet Microbiol 139, 147–152.[CrossRef] [Google Scholar]
  28. van Hemert, F. J., Lukashov, V. V. & Berkhout, B.(2007). Different rates of (non-)synonymous mutations in astrovirus genes; correlation with gene function. Virol J 4, 25.[CrossRef] [Google Scholar]
  29. van Leeuwen, M., Williams, M. M., Koraka, P., Simon, J. H., Smits, S. L. & Osterhaus, A. D.(2010). Human picobirnaviruses identified by molecular screening of diarrhoea samples. J Clin Microbiol 48, 1787–1794.[CrossRef] [Google Scholar]
  30. Zhu, H. C., Chu, D. K., Liu, W., Dong, B. Q., Zhang, S. Y., Zhang, J. X., Li, L. F., Vijaykrishna, D., Smith, G. J. & other authors(2009). Detection of diverse astroviruses from bats in China. J Gen Virol 90, 883–887.[CrossRef] [Google Scholar]
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