Isolation of β-globin-related genes from a human cosmid library

doi:10.1016/0378-1119(81)90028-7

Gene

Volume 13, Issue 3, April 1981, Pages 227-237

https://doi.org/10.1016/0378-1119(81)90028-7 Get rights and content

Abstract

A human gene library was constructed using an improved cloning technique for cosmid vectors. Human placental DNA was partially digested with restriction endonuclease Mboi, size-fractionated and ligated to BamHI-cut and phosphatase-treated cosmid vector pJB8. After packaging in λ phage particles, the recombinant DNA was transduced into Escherichia coli 1400 or HB101 followed by selection on ampicillin for recombinant E. coli. 150000 recombinant-DNA-containing colonies were screened for the presence of the human β-globin related genes. Five recombinants were isolated containing the human β-globin locus and encompassing approx. 70 kb of human DNA.

References (20)

R. Bernards et al.
Physical mapping of the globin gene deletion in (δβ)^o-thalassaemia
Gene
(1979)
H.W. Boyer et al.
A complementation analysis of the restriction and modification of DNA in Escherichia coli
J. Mol. Biol.
(1969)
M. Feiss et al.
Packaging of the bacteriophage λ chromosome: Effect of chromosome length
Virology
(1977)
R.A. Flavell et al.
Analysis of the β-δ-globin gene loci in normal and Hb Lepore DNA: Direct determination of gene linkage and intergene distance
Cell
(1978)
E.F. Fritsch et al.
Molecular cloning and characterization of the human β-like globin gene cluster
Cell
(1980)
D. Hanahan et al.
Plasmid screening at high colony density
Gene
(1980)
R.J. Roberts
Restriction and modification enzymes and their recognition sequences
Gene
(1980)
R. Bernards et al.
Physical mapping of the globin gene deletion in hereditary persistance of foetal haemoglobin (HPFH)
Nucl. Acids Res.
(1980)
H.C. Birnboim et al.
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Nucl. Acids Res.
(1979)
B. Cami et al.
Screening of cloned recombinant DNA in bacteria by in situ colony hybridization
Nucl. Acids Res.
(1978)

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Abstract

References (20)

Physical mapping of the globin gene deletion in (δβ)^o-thalassaemia

Gene

A complementation analysis of the restriction and modification of DNA in Escherichia coli

J. Mol. Biol.

Packaging of the bacteriophage λ chromosome: Effect of chromosome length

Virology

Analysis of the β-δ-globin gene loci in normal and Hb Lepore DNA: Direct determination of gene linkage and intergene distance

Cell

Molecular cloning and characterization of the human β-like globin gene cluster

Cell

Plasmid screening at high colony density

Gene

Restriction and modification enzymes and their recognition sequences

Gene

Physical mapping of the globin gene deletion in hereditary persistance of foetal haemoglobin (HPFH)

Nucl. Acids Res.

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

Nucl. Acids Res.

Screening of cloned recombinant DNA in bacteria by in situ colony hybridization

Nucl. Acids Res.

Cited by (0)