Cell
Volume 44, Issue 6, 28 March 1986, Pages 913-923
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Article
Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD10

https://doi.org/10.1016/0092-8674(86)90014-0Get rights and content

Abstract

The human excision repair gene ERCC-1 was cloned after DNA mediated gene transfer to the CHO mutant 43-3B, which is sensitive to ultraviolet light and mitomycin-C. We describe the cloning and sequence analysis of the ERCC-1 cDNA and partial characterization of the gene. ERCC-1 has a size of 15 kb and is located on human chromosome 19. The ERCC-1 precursor RNA is subject to alternative splicing of an internal 72 bp coding exon. Only the cDNA of the larger 1.1 kb transcript, encoding a protein of 297 amino acids, was able to confer resistance to ultraviolet light and mitomycin-C on 43-3B cells. Significant amino acid sequence homology was found between the ERCC-1 gene product and the yeast excision repair protein RAD10. The most homologous region displayed structural homology with DNA binding domains of various polypeptides.

References (49)

  • R.D. Wood et al.

    Repair capability and the cellular age response for killing and mutation induction after UV

    Mutation Res.

    (1982)
  • S.M. Berget

    Are U4 small nuclear ribonucleoproteins involved in polyadenylation?

    Nature

    (1984)
  • J.D. Brook et al.

    A somatic cell hybrid panel for chromosome 19 localization of known genes and RFLP's and orientation of the linkage group

    Cytogen. Cell Gen.

    (1985)
  • P.Y. Chou et al.

    Prediction of protein formation

    Biochemistry

    (1974)
  • E.A. de Weerd-Kastelein et al.

    Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization

    Nature New Biol.

    (1972)
  • J. de Wit et al.

    Assignment of structural β-galactosidase loci to human chromosome 3 and 22

    Hum. Genet.

    (1979)
  • E.C. Friedberg

    DNA Repair

    (1985)
  • A.H.M. Geurts van Kessel et al.

    Characterization of the Philadelphia chromosome by gene mapping

    Cytogenet. Cell Genet.

    (1981)
  • C. Gorman et al.

    High efficiency DNA-mediated transformation of primate cells

    Science

    (1983)
  • R.H. Haynes et al.

    DNA repair and mutagenesis in yeast

  • M. Kozak

    Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs

    Nucl. Acids Res.

    (1984)
  • K.H. Kraemer

    Heritable diseases with increased sensitivity to cellular injury

  • A. Laughon et al.

    Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins

    Nature

    (1984)
  • M.A. Maclnnes et al.

    DNA-mediated cotransfer of excision repair capacity and drug resistance into Chinese hamster ovary cell line UV-135

    Mol. Cell. Biol.

    (1984)
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