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The isolation and partial characterization of a highly pathogenic herpesvirus from the harbor seal(Phoca vitulina)

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This report describes the first isolation and partial characterization of a herpesvirus from the harbor seal(Phoca vitulina). The virus was isolated during a disease outbreak in a group of young seals nursed in an seal orphanage in The Netherlands. Almost half of the seals died with symptoms of acute pneumonia and focal hepatitis and the virus was isolated of organs of most of the dead animals. Seven out of ten seals of which paired serum samples were obtained showed seroconversion in a virus neutralization test during this outbreak. The virus was tentatively characterized as a herpesvirus (seal herpesvirus: SeHV or phocid herpesvirus 1) on the basis of its characteristic morphology in electron microscopy, buoyant density in sucrose, sensitivity to ether and heat treatment and its antigenic relationship with other probable members of the Alphaherpesvirinae subfamily. The virus caused cytopathic changes within 24 hours after inoculation in seal kidney cells, consisting of a focal rounding of cells and syncytium formation. No cytopathic changes were observed in the cells of nine other mammalian species tested.

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References

  1. Andrewes, C., Pereira, H. G., Wildy, P. (eds.): Viruses of Vertebrates, 4th ed. London: Ballière Tindall 1978.

    Google Scholar 

  2. Dawson, R. M. C., Elliot, D. C., Elliot, W. H., Jones, K. M. (eds.), Data for Biochemical Research. London: Oxford University Press 1969.

    Google Scholar 

  3. Geraci, J. R., Aubin, D. J. St., Barker, J. K., Webster, R. G., Hinshaw, V. S., Bean, W. J., Ruhnke, H. L., Prescott, J. H., Early, G., Baker, A. S., Madoff, S., Schooley, R. T.: Mass mortality of harbor seals: Pneumonia associated with Influenza A virus. Science215, 1129–1130 (1982).

    Google Scholar 

  4. Honess, R. W., Watson, D. H.: Unity and diversity in the Herpesviruses. J. gen. Virol.37, 15–37 (1977).

    Google Scholar 

  5. Osterhaus, A., Berghuis de Vries, J., Steur, K.: Antiviral antibodies in dogs in The Netherlands. Zbl. Vet. Met. B.24, 123–133 (1977).

    Google Scholar 

  6. Osterhaus, A. D. M. E., van Steenis, G., de Kreek, P.: Isolation of a virus closely related to feline panleukopenia virus from dogs with diarrhea. Zbl. Vet. Med.B 27, 11–21 (1980).

    Google Scholar 

  7. Roizman, B., Carmichael, L. E., Deinhardt, F., de The, G., Nahmias, A. J., Plowright, W., Rapp, F., Sheldrick, P., Takahashi, M., Wolf, K.: Herpesviridae. Intervirol.16, 201–217 (1981).

    Google Scholar 

  8. Sober, H. A. (ed.): Handbook of biochemistry. Selected Data for Molecular Biology, 2nd ed. Cleveland: The Chemical Rubber Co. 1970.

    Google Scholar 

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Osterhaus, A.D.M.E., Yang, H., Spijkers, H.E.M. et al. The isolation and partial characterization of a highly pathogenic herpesvirus from the harbor seal(Phoca vitulina) . Archives of Virology 86, 239–251 (1985). https://doi.org/10.1007/BF01309828

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  • DOI: https://doi.org/10.1007/BF01309828

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