Elsevier

Vaccine

Volume 10, Issue 3, 1992, Pages 192-197
Vaccine

Paper
An immune stimulating complex (ISCOM) subunit rabies vaccine protects dogs and mice against street rabies challenge

https://doi.org/10.1016/0264-410X(92)90011-8Get rights and content

Abstract

Dogs and mice were immunized with either a rabies glycoprotein subunit vaccine incorporated into an immune stimulating complex (ISCOM) or a commercial human diploid cell vaccine (HDCV) prepared from a Pitman Moore (PM) rabies vaccine strain. Pre-exposure vaccination of mice with two intraperitoneal (i.p.) doses of 360 ng ISCOM or 0.5 ml HDCV protected 95% (3840) and 90% (3640) of mice, respectively, against a lethal intracerebral (i.c.) dose with challenge virus strain (CVS). One 360 ng i.p. dose of ISCOM protected 87.5% (3540) of mice against i.c. challenge with CVS. Three groups of five dogs were vaccinated intramuscularly (i.m.) with 730 ng of rabies ISCOM prepared from either the PM or the CVS rabies strains, and they resisted lethal street rabies challenge. Postexposure treatment of mice with three or four 120 ng i.m. doses of ISCOM protected 90% (2730) and 94% (4548), respectively, of mice inoculated in the footpad with street rabies virus, but three doses of HDCV conferred no protection. When four doses of HDCV were administered postexposure, 78% (3241) of the mice died of anaphylactic shock; 21% (1152) of mice had already died of rabies 4 days after the third vaccine dose was administered.

References (65)

  • S. Seligmann

    Semple-type vaccine

  • P. Lepine

    Fermi-type vaccine

  • M.K. Karakujumcan et al.

    Suckling Rat vaccine

  • E. Fuenzalida

    Suckling mouse brain vaccine

  • H. Koprowski et al.

    Studies on chick embryo adapted rabies virus

  • H.M. Powell et al.

    Action of street rabies vaccine derived from embryonated duck eggs against street rabies virus

  • J.M. Hoskins

    Duck-embryo vaccine

  • M. Bahmanyar et al.

    Successful protection of humans exposed to rabies infection

  • L. Fang-Tao et al.

    Study of the protective efficacy of primary hamster kidney cell rabies vaccine

    J. Infect. Dis.

    (1986)
  • P. Fournier et al.

    New vaccine produced from rabies cultivated on vero cells

  • K. Habel

    Ultraviolet-light irradiation for inactivation of vaccine

  • W.H. Wunner et al.

    Rabies subunit vaccines

    J. Gen. Virol.

    (1983)
  • J. Crick et al.

    Viral subunit rabies vaccination

    Nature

    (1969)
  • L. Thibodeau et al.

    Immunosome technology: an approach to efficient and safe influenza and rabies vaccine

  • W.H. Tordo et al.

    Walking along the rabies genome: Is the large G-L intergenic region remnant gene?

  • W.H. Wunner et al.

    The molecular biology of rabies viruses

    Rev. Infect. Dis.

    (1988)
  • H.K. Clark

    Systems for assay and growth of rhabdoviruses

  • T.J. Wiktor et al.

    In vitro evidence of cell-mediated immunity after exposure of mice to both live and inactivated rabies virus

  • H. Tsiang et al.

    In vitro detection of cell-mediated immunity in street rabies virus infection in mice

    J. Gen. Virol.

    (1980)
  • T.J. Wiktor et al.

    Antigenic variants of rabies virus

    J. Exp. Med.

    (1980)
  • T.J. Wiktor et al.

    Antigenic properties of rabies components

    J. Immunol.

    (1973)
  • R.K. Sikes et al.

    Effective protection of monkeys against death from street virus by post exposure administration of tissue culture rabies vaccine

    Bull. WHO

    (1971)
  • Cited by (22)

    • Recombinant glycoprotein based vaccine for Chandipura virus infection

      2009, Vaccine
      Citation Excerpt :

      The two mice not protected by the vaccine died on 4 (1 μg) and 7 (2 μg) days post-infection; with 500 ng rGp five mice died during an interval of 2–7 days post-infection, similar to unimmunized controls. It is pertinent to note here that rabies glycoprotein sub-unit vaccine at as low dose as 360 ng could offer protection to 87.5% immunized mice after intracerebral challenge [24]. When anti-CHPV antibody titers as determined by NT or ELISA were compared with the protection offered against the intracerebral challenge of CHPV in mice, it was estimated that an ELISA titer of 1:40 or NT titer of 1:20 could be considered protective.

    • RABIES VIRUS

      2009, Feigin and Cherry's Textbook of Pediatric Infectious Diseases, Sixth Edition
    View all citing articles on Scopus
    View full text