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Home / Journal of Nanoscience and Nanotechnology, Volume 17, Number 2

Purification of Biotinylated Proteins Using Single Walled Carbon Nanotube-Streptavidin Complexes

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In this study, Single walled carbon nanotube (SWNT)-streptavidin complexes were used to capture and purify biotinylated proteins, including bio-GFP and bio-DBS using a pull-down method. The purification conditions were systematically studied, including surface blocking of SWNT using chicken egg albumin (CEA), the ratio of SWNT-streptavidin complexes to the cell lysate, as well as the centrifugation speed. Optimization of the protein purification using SWNT-streptavidin complexes shows the possibility of carbon nanotubes as a promising candidate for protein purification applications. The SWNT-streptavidin could be used as a scaffold to analyze protein structure directly by cryo-transmission electron microscopy, which provides better understanding in protein–protein interactions and biological processes.

Keywords: Biotinylated Proteins; Chick Egg Albumin; SWNT-Streptavidin

Document Type: Research Article

Affiliations: 1: School of Pharmaceutical Engineering and Life Science, School of Nursing, Jiangsu Collaborative Innovation Center of Photovoltaic Science and Engineering, Department of Materials Science and Engineering, Changzhou University, Changzhou, 213164, China 2: Department of Biophysical Structural Chemistry, Leiden University, 2333CC, The Netherlands 3: School of Pharmaceutical Engineering and Life Science, School of Nursing, Jiangsu Collaborative Innovation Center of Photovoltaic Science and Engineering, Department of Materials Science and Engineering, Changzhou University, Changzhou, 213164, China 4: Department of Cell Biology and Genetics, Erasmus MC, 3000 CA, Rotterdam, The Netherlands 5: State Key Laboratory of Natural Medicines, Department of Organic Chemistry, China Pharmaceutical University, Nanjing 210009, China 6: Department of Physics, College of Sciences, Tianjin University of Science and Technology, Tianjin, 300457, China 7: C-CINA, Biozentrum Basel, Switzerland; LBR, Paul Scherrer Institute, Villigen CH-4003, Switzerland

Publication date: 01 February 2017

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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