Intracellularly expressed single-domain antibody against p15 matrix protein prevents the production of porcine retroviruses
The presence of porcine endogenous retroviruses presents a potential risk of transmission of infectious diseases (xenozoonosis) if tissues and organs from genetically modified pigs are to be used in xenotransplantation. Here, we report that intracellular expression of a llama single-domain antibody against p15, the matrix domain protein of the porcine endogenous retrovirus Gag polyprotein, blocks retrovirus production, providing the possibility of eliminating the risk of infection in xenotransplantation.
|Keywords||Amino Acid Sequence, Animals, Antibodies, Viral/*physiology, Antibody Affinity, Endogenous Retroviruses/*physiology, Epitope Mapping, Gene Products, gag/*antagonists & inhibitors/immunology, Humans, Immune Sera/immunology, Immunoglobulin Heavy Chains/physiology, Immunoglobulin Variable Region/physiology, Molecular Sequence Data, Research Support, Non-U.S. Gov't, Retroviridae Infections/*prevention & control, Swine/*virology, Transplantation, Heterologous/*adverse effects, Viral Matrix Proteins/*antagonists & inhibitors/immunology, Virus Replication|
Dekker, S., Toussaint, W., Panayotou, G., Visser, P., Drabek, D.D., Grosveld, F.G., & de Wit, T.P.M.. (2003). Intracellularly expressed single-domain antibody against p15 matrix protein prevents the production of porcine retroviruses. Journal of Virology. Retrieved from http://hdl.handle.net/1765/10237