2000
Erythroid overexpression of C/EBPgamma in transgenic mice affects gamma-globin expression and fetal liver erythropoiesis.
Publication
Publication
EMBO Journal , Volume 19 - Issue 21 p. 5856- 5863
The CCAAT boxes of the beta-like globin genes interact with three proteins: NF-Y, GATA-1 and NFE-6. We demonstrate that NFE-6 contains C/EBPgamma, and address its role in globin gene regulation by erythroid overexpression of C/EBPgamma, and a dominant-negative form C/EBPgammaDeltaB, in mice. Elevated levels of C/EBPgamma, but not C/EBPgammaDeltaB, increase expression of the (fetal) gamma-globin relative to the (adult) beta-globin gene. Interestingly, fetal liver erythropoiesis is ablated when the C/EBPgamma and C/EBPgammaDeltaB levels are further increased in homozygous transgenics. We suggest that targeted expression of dominant-negative leucine zipper proteins is a generally applicable approach to ablate specific tissues in mice.
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doi.org/10.1093/emboj/19.21.5856, hdl.handle.net/1765/12904 | |
EMBO Journal | |
Organisation | Erasmus MC: University Medical Center Rotterdam |
Zafarana, G., Rottier, R., Grosveld, F., & Philipsen, S. (2000). Erythroid overexpression of C/EBPgamma in transgenic mice affects gamma-globin expression and fetal liver erythropoiesis. EMBO Journal, 19(21), 5856–5863. doi:10.1093/emboj/19.21.5856 |