The G-protein gamma subunit gpc-1 of the nematode C.elegans is involved in taste adaptation.
Caenorhabditis elegans has two heterotrimeric G-protein gamma subunits, gpc-1 and gpc-2. Although GPC-1 is specifically expressed in sensory neurons, it is not essential for the detection of odorants or salts. To test whether GPC-1 is involved in sensory plasticity, we developed a water soluble compound adaptation assay. The behaviour of wild-type animals in this assay confirms that prolonged exposure to salts can abolish chemo-attraction to these compounds. This process is time and concentration dependent, partly salt specific and reversible. In contrast, gpc-1 mutant animals show clear deficits in their ability to adapt to NaAc, NaCl and NH4Cl, but normal wild-type adaptation to odorants. Two other loci previously implicated in odorant adaptation, adp-1 and osm-9, are also involved in adaptation to salts. Our finding that G proteins, OSM-9 and ADP-1 are involved in taste adaptation offer the first molecular insight into this process.
|Keywords||*GTP-Binding Protein gamma Subunits, Acetates/pharmacology, Adaptation, Physiological/genetics/*physiology, Ammonium Chloride/pharmacology, Animals, Caenorhabditis elegans Proteins/biosynthesis/genetics/*physiology, Caenorhabditis elegans/drug effects/genetics/*physiology, Chemotaxis/physiology, Heterotrimeric GTP-Binding Proteins/biosynthesis/genetics/*physiology, Ion Channels/genetics/physiology, Nerve Tissue Proteins/biosynthesis/genetics/*physiology, Osmolar Concentration, Research Support, Non-U.S. Gov't, Smell/physiology, Sodium Acetate/pharmacology, Sodium Chloride/pharmacology, Taste/genetics/*physiology, Transient Receptor Potential Channels|
|Persistent URL||dx.doi.org/10.1093/emboj/21.5.986, hdl.handle.net/1765/13022|
Jansen, G., Weinkove, D., & Plasterk, R.A.. (2002). The G-protein gamma subunit gpc-1 of the nematode C.elegans is involved in taste adaptation.. EMBO Journal, 21(5), 986–994. doi:10.1093/emboj/21.5.986