Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCC mec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.

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Keywords Staphylococcus aureus
Persistent URL dx.doi.org/10.1007/s10096-009-0816-3, hdl.handle.net/1765/17240
van Wamel, W.J.B, Hansenová Maňásková, S, Fluit, A, Verbrugh, H.A, de Neeling, A.J, van Duijkeren, E, & van Belkum, A.F. (2010). Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398. European Journal of Clinical Microbiology & Infectious Diseases: an international journal on pathogenesis, diagnosis, epidemiology, therapy, and prevention of infectious diseases, 29(1), 119–122. doi:10.1007/s10096-009-0816-3