NF1 inactivation occurs in specific human cancers, including juvenile myelomonocytic leukemia, an aggressive myeloproliferative disorder of childhood. However, evidence suggests that Nf1 loss alone does not cause leukemia.We therefore hypothesized that inactivation of the Nf1 tumor suppressor gene requires cooperating mutations to cause acute leukemia. To search for candidate genes that cooperate with Nf1 deficiency in leukemogenesis, we performed a forward genetic screen using retroviral insertion mutagenesis in Nf1 mutant mice. We identified 43 common proviral insertion sites that contain candidate genes involved in leukemogenesis. One of these genes, Bc111a, confers a growth advantage in cultured Nf1 mutant hematopoietic cells and causes early onset of leukemia of either myeloid or lymphoid lineage in mice when expressed in Nf1-deficient bone marrow. Bc111a-expressing cells display compromised p21Cip1 induction, suggesting that Bc111a's oncogenic effects are mediated, in part, through suppression of p21Cip1. Importantly, Bc111a is expressed in human chronic myelomonocytic leukemia and juvenile myelomonocytic leukemia samples. A subset of AML patients, who had poor outcomes, of 16 clusters, displayed high levels of BCL11A in leukemic cells. These findings suggest that deregulated Bc111a cooperates with Nf1 in leukemogenesis, and a therapeutic strategy targeting the BCL11A pathway may prove beneficial in the treatment of leukemia.

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Keywords BCL11A gene, BCL11A protein, Bcl11a protein, CDKN1A protein, Cdkn1a protein, Retrovirus, acute granulocytic leukemia, acute lymphocytic leukemia, animal, animal cell, animal experiment, animal model, article, bone marrow, carcinogenicity, carrier protein, cell culture, cell line, cell lineage, chronic myeloid leukemia, chronic myelomonocytic leukemia, controlled study, cyclin dependent kinase inhibitor 1, cyclin dependent kinase inhibitor 1A, gene, gene expression, gene expression regulation, gene inactivation, gene insertion, gene loss, gene mutation, gene overexpression, genetic screening, genetics, hematopoietic cell, human, juvenile myelomonocytic leukemia, leukemia cell, leukemogenesis, lymphoid cell, metabolism, methodology, mouse, mouse mutant, mutagenesis, mutant, neurofibromin, nonhuman, nuclear protein, priority journal, transgenic mouse, tumor suppressor gene
Persistent URL dx.doi.org/10.1182/blood-2008-03-144436, hdl.handle.net/1765/19338
Citation
Yin, B., Delwel, H.R., Valk, P.J.M., Wallace, M.R., Loh, M.L., Shannon, K.M., & Largaespada, D.A.. (2009). A retroviral mutagenesis screen reveals strong cooperation between Bc111a overexpression and loss of the Nf1 tumor suppressor gene. Blood, 113(5), 1075–1085. doi:10.1182/blood-2008-03-144436