Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asunción, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97% of the total isolates carried the esp gene. Three Tn. 1546 variants were found, including a new lineage containing an IS. Efa5 insertion in an existing IS1251 element.

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doi.org/10.1111/j.1469-0691.2009.02898.x, hdl.handle.net/1765/20323
Clinical Microbiology and Infection
Erasmus MC: University Medical Center Rotterdam

Khan, M. A., Northwood, J. B., Loor, R. G. J., Tholen, A. T. R., Riera, E., Falcón, M., … Hays, J. (2010). High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay. Clinical Microbiology and Infection (Vol. 16, pp. 624–627). doi:10.1111/j.1469-0691.2009.02898.x