Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asunción, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97% of the total isolates carried the esp gene. Three Tn. 1546 variants were found, including a new lineage containing an IS. Efa5 insertion in an existing IS1251 element.

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Keywords Bacterial genotypes, Insertion sequences, Multilocus sequence typing, Transposons, Vancomycin-resistant Enterococci
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Journal Clinical Microbiology and Infection
Khan, M.A, Northwood, J.B, Loor, R.G.J, Tholen, A.T.R, Riera, E, Falcón, M, … Hays, J.P. (2010). High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay. Clinical Microbiology and Infection (Vol. 16, pp. 624–627). doi:10.1111/j.1469-0691.2009.02898.x