ATP-dependent chromatin-remodeling complexes (remodelers) are essential regulators of chromatin structure and gene transcription. How remodelers can act in a gene-selective manner has remained enigmatic. A yeast two-hybrid screen for proteins binding the Drosophila transcription factor Tramtrack69 (TTK69) identified MEP1. Proteomic characterization revealed that MEP1 is a tightly associated subunit of the NuRD remodeler, harboring the Mi2 enzymatic core ATPase. In addition, we identified the fly homolog of human Deleted in oral cancer 1 (DOC1), also known as CDK2-associated protein 1 (CDK2AP1), as a bona fide NuRD subunit. Biochemical and genetic assays supported the functional association between MEP1, Mi2, and TTK69. Genomewide expression analysis established that TTK69, MEP1, and Mi2 cooperate closely to control transcription. The TTK69 transcriptome profile correlates poorly with remodelers other than NuRD, emphasizing the selectivity of remodeler action. On the genes examined, TTK69 is able to bind chromatin in the absence of NuRD, but targeting of NuRD is dependent on TTK69. Thus, there appears to be a hierarchical relationship in which transcription factor binding precedes remodeler recruitment.

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Keywords Drosophila, article, chemical analysis, chromatin assembly and disassembly, cyclin dependent kinase 2, gene expression, genetic analysis, hybridization, nonhuman, priority journal, protein MEP 1, protein analysis, protein binding, protein doc 1, protein nurd, protein protein interaction, protein ttk 69, proteomics, screening, transcription factor, unclassified drug, yeast
Persistent URL dx.doi.org/10.1128/MCB.00266-10, hdl.handle.net/1765/21317
Citation
Reddy, B.A, Bajpe, P.K, Bassett, A, Moshkin, Y.M, Kozhevnikova, E, Bezstarosti, K, … Verrijzer, C.P. (2010). Drosophila transcription factor tramtrack69 binds MEP1 to recruit the chromatin remodeler NuRD. Molecular and Cellular Biology, 30(21), 5234–5244. doi:10.1128/MCB.00266-10