Isolation and characterization of the human collagen α1(I)-like gene from a cosmid library.
We have isolated a human collagen alpha 1(I)-like gene from a cosmid library. The clone which contains 37kb of human DNA has been shown to contain this gene by DNA sequencing, hybrid arrest and hybrid selection assays and Northern blot hybridizations. The collagen gene sequence extends through most of the cloned DNA and must, therefore, be at least 35kb in length.
|Keywords||*DNA/*genetics, *Genes, Structural, *Plasmids, 0 (Plasmids), 0 (RNA, Messenger), 9007-34-5 (Collagen), 9007-49-2 (dna), Base Sequence, Collagen/*genetics, DNA Restriction Enzymes, EC 3.1.21 (DNA Restriction Enzymes), Human, Nucleic Acid Hybridization, RNA, Messenger/genetics, Support, Non-U.S. Gov't, Transcription, Genetic|
Weiss, E.H., Cheah, K.S.E., Grosveld, F.G., Dahl, H.H.M., Solomon, E., & Flavell, R.A.. (1994). Isolation and characterization of the human collagen α1(I)-like gene from a cosmid library.. Nucleic Acids Research, 10(8), 1981–1994. Retrieved from http://hdl.handle.net/1765/2352