Analysis of cosmid clones of nuclear DNA from Trypanosome brucei shows that the genes for variant surface glycoproteins are clustered in the genome.
Trypanosoma brucei contains more than a hundred genes coding for the different variant surface glycoproteins (VSGs). Activation of some of these genes involves the duplication of the gene (the basic copy or BC) and transposition of the duplicate to an expression site (yielding the expression-linked copy or ELC). We have cloned large fragments of genomic DNA in cosmid vectors in Escherichia coli. Cosmids containing the BCs of genes 117, 118 and 121 were readily obtained, but DNA containing the ELCs was strongly selected against in the cosmid and plasmid cloning systems used. We have analysed the distribution of VSG genes in the genome using probes for the sequences at the edges of the transposed segment which are partially homologous among these genes. In genomic cosmid clone banks, about 9% of all colonies hybridize with probes from the 5'- and 3'-edges of the transposed segment, showing that these sequences are linked in the genome. Moreover, the 117 and 118 BC cosmids contain several additional putative VSG genes in tandem, as deduced from hybridization and sequence analyses. We conclude that the VSG genes are highly clustered and share common sequences at the borders of the transposed segment.
|Keywords||0 (Glycoproteins), 0 (Membrane Proteins), 0 (Plasmids), Animals, Cell Nucleus/metabolism, Cloning, Molecular, DNA Restriction Enzymes, EC 3.1.21 (DNA Restriction Enzymes), Genes, Structural, Glycoproteins/genetics, Membrane Proteins/genetics, Nucleic Acid Hybridization, Plasmids, Support, Non-U.S. Gov't, Trypanosoma brucei brucei/metabolism, Variation (Genetics)|
Valerio, D., de Lange, T., Borst, P., Grosveld, F.G., & van der Ploeg, L.H.T.. (1982). Analysis of cosmid clones of nuclear DNA from Trypanosome brucei shows that the genes for variant surface glycoproteins are clustered in the genome.. Nucleic Acids Research, 10(19), 5905–5924. Retrieved from http://hdl.handle.net/1765/2357