Isolation of human oncogene sequences (v-fes homologue) from a cosmid library.
To define the human homolog (or homologs) of transforming sequences (v-fes gene) common to Gardner (GA) and Snyder Theilen (ST) isolates of feline sarcoma virus (FeSV), a representative library of human lung carcinoma DNA in a cosmid vector system was constructed. Three cosmid clones were isolated containing GA/ST FeSV v-fes homologous cellular sequences, within 32- to 42-kilobase cellular inserts representing 56 kilobases of contiguous human cellular DNA. Sequences both homologous to, and colinear with, GA or ST FeSV v-fes are distributed discontinuously over a region of up to 9.5 kilobases and contain a minimum of three regions of nonhomology representing probable introns. A thymidine kinase selection system was used to show that, upon transfection to RAT-2 cells, the human c-fes sequence lacked detectable transforming activity.
|Keywords||0 (DNA, Recombinant), Bacteriophage lambda/genetics, Cell Transformation, Viral, Cloning, Molecular/methods, DNA Restriction Enzymes/diagnostic use, DNA, Recombinant, EC 3.1.21 (DNA Restriction Enzymes), Escherichia coli/genetics, Genes, Viral, Human, Retroviridae/genetics, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S.|
Groffen, J., Heisterkamp, N., Grosveld, F.G., van de Ven, W., & Stephenson, J.R.. (1982). Isolation of human oncogene sequences (v-fes homologue) from a cosmid library.. Science, 216, 1136–1138. Retrieved from http://hdl.handle.net/1765/2358