The sequence GGCmCGG is resistant to MspI cleavage
MspI essentially fails to cut the sequence GGCmCGG at enzyme concentrations which give total digestion of CCGG, CmCGG and GGCCGG sites. This result explains why certain sites in mammalian DNA are resistant to both MspI and HpaII and shows that this results from an idiosynchracy of MspI rather than a novel form of DNA methylation at this site in mammalian cells.
|Keywords||9004-22-2 (Globins), 9007-49-2 (dna), Animals, Base Sequence, Cloning, Molecular, DNA Restriction Enzymes/metabolism, Deoxyribonuclease HpaII, Dna, EC 18.104.22.168 (Thymidine Kinase), EC 3.1.21 (DNA Restriction Enzymes), EC 3.1.21.- (Deoxyribonuclease HpaII), Genes, Structural, Globins/genetics, Human, L Cells (Cell Line)/enzymology, Methylation, Mice, Nucleic Acid Hybridization, Substrate Specificity, Support, Non-U.S. Gov't, Thymidine Kinase/deficiency|
Busslinger, M., Wright, S., Grosveld, F.G., Flavell, R.A., & de Boer, E.. (1983). The sequence GGCmCGG is resistant to MspI cleavage. Nucleic Acids Research, 11(11), 3559–3569. Retrieved from http://hdl.handle.net/1765/2366