Cloning of a cDNA encoding the smallest neurofilament protein from the rat
We have cloned a cDNA coding for the smallest rat neurofilament protein. The cDNA is 861 nucleotides long coding for 287 amino acids from the internal alpha-helical region and the carboxy-terminal tail domain of the neurofilament protein. Comparison of the porcine, mouse and rat neurofilament protein sequences shows that the protein is highly conserved (greater than 93% identity). Blot analysis indicates that the cDNA is derived from a single neurofilament gene that codes for two different poly(A)+ mRNA species.
|Keywords||0 (Intermediate Filament Proteins), 0 (Nerve Tissue Proteins), 0 (Neurofilament Proteins), 0 (Oligodeoxyribonucleotides), 0 (RNA, Messenger), 9007-49-2 (dna), Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA/genetics, Genes, Structural, Intermediate Filament Proteins/*genetics, Molecular Weight, Nerve Tissue Proteins/*genetics, Neurofilament Proteins, Oligodeoxyribonucleotides/diagnostic use, RNA, Messenger/genetics, Rats, Support, Non-U.S. Gov't, Transcription, Genetic|
Julien, J-P., Ramachadran, K., & Grosveld, F.G.. (1985). Cloning of a cDNA encoding the smallest neurofilament protein from the rat. B B A - Proteins and Proteomics, 825, 398–404. Retrieved from http://hdl.handle.net/1765/2389