Specific detection of neuronal cell bodies: in situ hybridization with a biotin-labelled neurofilament cDNA probe.
We have used a biotinylated, 300-nucleotide cDNA probe which encodes the 68,000 MW neurofilament protein to detect neurofilament-specific mRNA in situ. The neurofilament message specifically demonstrates the neuronal cell bodies, in contrast to the usual antibody staining which detects their neurites. The hybridization is detected only in neuronal structures. Consequently, detection of the biotinylated neurofilament DNA probe by silver-intensified streptavidin-gold can be specifically used to identify neuronal cell bodies.
|Keywords||*Genetic Markers, *Nucleic Acid Hybridization, 0 (Bacterial Proteins), 0 (Genetic Markers), 0 (Intermediate Filament Proteins), 58-85-5 (Biotin), 7440-57-5 (Gold), 9007-49-2 (dna), 9013-20-1 (Streptavidin), Animals, Bacterial Proteins/diagnostic use, Biotin/*diagnostic use, Brain Chemistry, Brain/cytology, Cytoskeleton/*analysis, DNA/*diagnostic use, Gold/diagnostic use, Intermediate Filament Proteins/*analysis/genetics, Intermediate Filaments/*analysis, Neurons/*analysis/ultrastructure, Rats, Rats, Inbred Strains, Streptavidin|
Liesi, P., Julien, J-P., Vilja, P., Grosveld, F.G., & Rechardt, L.. (1986). Specific detection of neuronal cell bodies: in situ hybridization with a biotin-labelled neurofilament cDNA probe.. Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 34, 923–926. Retrieved from http://hdl.handle.net/1765/2399