Progress towards construction of a total restriction fragment map of a human chromosome.
We present an approach to the construction of an overlapping restriction fragment map of a single human chromosome. A genomic cosmid library genome was constructed from a mouse-human hybrid cell line containing chromosome 17 as its only human genetic component. Cosmids containing human inserts were isolated by hybridisation to a human Alu sequence. DNAs from ninety-six randomly chosen cosmids were digested with either EcoRI or HindIII, end-labelled with 35S-dATP and analysed using agarose gel electrophoresis. Comparison of the restriction fragment patterns revealed two pairs of overlapping clones, that were confirmed by cross-hybridization of the overlapping fragments. The two pairs of cosmids both mapped to human chromosome 17, as shown by hybridization to a panel of somatic cell hybrids. These data demonstrate that the generation of an overlapping cosmid map along a human chromosome is feasible, representing an intermediate step towards the complete sequencing of a human chromosome.
|Keywords||*Chromosomes, Human, Pair 17, *DNA Restriction Enzymes, 0 (Cosmids), Animals, Cell Line, Chromosome Mapping, Cosmids, EC 3.1.21 (DNA Restriction Enzymes), Human, Hybrid Cells/cytology, Mice, Support, Non-U.S. Gov't|
Vissing, H., Grosveld, F.G., Solomon, E., Moore, G., Lench, N., Shennan, N., & Williamson, R.. (1987). Progress towards construction of a total restriction fragment map of a human chromosome.. Nucleic Acids Research, 15, 1363–1375. Retrieved from http://hdl.handle.net/1765/2403