T-cell independent Thy-1 allo-antibody response with the use of transgenic mice.
We have introduced a mouse Thy-1.1 gene into the germline of Thy-1.2 mice. The introduced gene was shown to be expressed at very high levels in thymocytes when compared with the endogenous gene. Transgenic thymocytes were shown to evoke a higher than normal primary anti-Thy-1.1 antibody response in plaque-forming cell (PFC) assays. This result suggests that a direct quantitative interaction of the Thy-1 antigen activates the B cell response.
|Keywords||0 (Antigens, Surface), 0 (Antigens, T-Independent), 0 (Antigens, Thy-1), 0 (Isoantibodies), Animals, Antigens, Surface/genetics/*immunology, Antigens, T-Independent/immunology, Antigens, Thy-1, B-Lymphocytes/*immunology, Cloning, Molecular, Gene Expression Regulation, Isoantibodies/biosynthesis, Mice, Mice, Inbred Strains, Support, Non-U.S. Gov't, T-Lymphocytes/*immunology, Thymus Gland/immunology, Tissue Distribution, Transfection|
Isobe, K-I., Kollias, G., Kolsto, A-B., & Grosveld, F.G.. (1986). T-cell independent Thy-1 allo-antibody response with the use of transgenic mice.. Journal of Immunology, 137, 2089–2092. Retrieved from http://hdl.handle.net/1765/2404