Differential regulation of a Thy-1 gene in transgenic mice.
We have generated Thy-1.1-transgenic Thy-1.2 mice to study the developmental expression of the Thy-1 gene in detail by transcriptional and immunological methods. In brain, the expression of the injected gene was identical to that of the endogenous gene in a tissue- and development-specific manner. In lymphoid tissue, the transferred gene was also expressed correctly in the early phases of T-cell lineage development; however, as the T cells matured, the transcription of the transferred gene, but not the endogenous gene, was suppressed. This result shows that different regulatory elements are used to express the Thy-1 gene in early and late lymphoid development.
|Keywords||*Gene Expression Regulation, 0 (Antigens, Surface), 0 (Antigens, Thy-1), 0 (RNA, Messenger), Age Factors, Animals, Antigens, Surface/biosynthesis/*genetics, Antigens, Thy-1, Brain/immunology, Crosses, Genetic, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, RNA, Messenger/analysis, Support, Non-U.S. Gov't, T-Lymphocytes/immunology, Transcription, Genetic|
Kollias, G., Spanopoulou, E., Grosveld, F.G., Ritter, M., Beech, J., & Morris, R.. (1987). Differential regulation of a Thy-1 gene in transgenic mice.. Proceedings of the National Academy of Sciences of the United States of America, 1492–1496. Retrieved from http://hdl.handle.net/1765/2411